Interactions of triglycerides with phospholipids: Incorporation into the bilayer structure and formation of emulsions

Journal Article · Mon Mar 20 23:00:00 EST 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00432a025· OSTI ID:5476745

Hamilton, J A ^[1]

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1. Boston Univ. School of Medicine, MA (USA)

Interactions of carbonyl {sup 13}C-enriched triacylglycerols (TG) with phospholipid bilayers were studied by {sup 13}C NMR spectroscopy. In spectra of DPPC vesicles with TG at 40-50{degree}C, both triolein (TO) and tripalmitin (TP) had narrow carbonyl resonances, indicative of rapid motions, and chemical shifts indicative of H bonding of the TG carbonyls with solvent (H{sub 2}O) at the aqueous interfaces of the vesicle bilayer. Below the phase transition temperature of the DPPC/TG vesicles, most phospholipid peaks broadened markedly. In DPPC vesicles with TP, the TP carbonyl peaks broadened beyond detection below the transition, whereas in vesicles with TO, the TO carbonyl peaks showed little change in line width or chemical shift and no change in the integrated intensity. These properties (extent of solubility in the PC surface, conformation, solvent accessibility, and molecular mobility) may be important for enzymatic hydrolysis and protein-mediated transfer of TG. In gel-phase DPPC, the molecular mobility of the TG depends on the nature of the TG acyl chains. In the DPPC/TG mixtures studied, attempts to incorporate TG in excess of the bilayer solubility resulted in production of emulsion particles. The significance of these results for TG metabolism is discussed.

• OSTI ID: 5476745
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 28:6; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
CALORIMETRY
CARBON 13
CARBON ISOTOPES
DEUTERIUM COMPOUNDS
ELECTRON MICROSCOPY
ESTERS
EVEN-ODD NUCLEI
HYDROGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MEMBRANES
MICROSCOPY
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHASE STUDIES
PHOSPHOLIPIDS
RESONANCE
SPECTRA
STABLE ISOTOPES
TRIGLYCERIDES