2,3,7,8 tetrachlorodibenzodioxin in fish from the Pigeon river of Eastern Tennessee: Its toxicity and mutagenicity as revealed by the Ames Salmonella Assay

Conference · Sun Apr 01 04:00:00 EDT 1990 · Journal of the Tennessee Academy of Science; (USA)

OSTI ID:5476698

Blevins, R D ^[1]

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1. East Tennessee State Univ., Johnson City (USA)

Levels of 2,3,7,8 tetrachlorodibenzodioxin (TCDD)were determined in both striated muscle (fillets) and whole body extracts of fish specimens harvested during a two year period (1987-1989) from the Pigeon River (between Hartford and Newport) of Eastern Tennessee. Whole body (wet weight) fish extract levels as high as 117 {mu}g/kg body weight and composite fish fillet (wet weight) extract levels as high 87 {mu}g/kg fillet weight were observed. Pure TCDD was found to be highly toxic to the Salmonella typhimurium strains TA97, TA98, TA100, TA102 and TA1535 at TCDD dosages which exceeded 825 ng/ml in the top agar of the Ames Salmonella assay. An 825 ng/ml TCDD dosage was not mutagenic to any of the tested Salmonella strains, (both with and without metabolic activation (S9) mix). However, when both acidic and alcohol fish extracts from the Pigeon River were tested for mutagenicity, several of the fish extracts were found to be mutagenic to Salmonella strains TA97, TA98, and TA100 (having mutagenic ratios which greatly exceeded the 2.5 {times} spontaneous ratio). These mutagenic extracts also demonstrated mutagenic dose-response curves. Other chemicals within the extracts as well as synergistic effects may account for the mutagenicity.

• OSTI ID: 5476698
• Report Number(s): CONF-8911221--
• Conference Information: Journal Name: Journal of the Tennessee Academy of Science; (USA) Journal Volume: 65:2
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AQUATIC ORGANISMS
BACTERIA
CHLORINE COMPOUNDS
CONTAMINATION
DEVELOPED COUNTRIES
DIOXIN
FEDERAL REGION IV
FISHES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
MUTAGEN SCREENING
NORTH AMERICA
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RIVERS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
STREAMS
SURFACE WATERS
TENNESSEE
USA
VERTEBRATES