In vitro effects of toxaphene on mitochondrial calcium ATPase and calcium uptake in selected rat tissues

Journal Article · Mon Feb 04 04:00:00 EST 1985 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(85)90254-1· OSTI ID:5474623

Trottman, C H; Rao, K S.P.; Morrow, W; Uzodinma, J E; Desaiah, D

In vitro effects of toxaphene on Ca/sup 2 +/-ATPase activity and /sup 45/Ca/sup 2 +/-uptake were studied in mitochondrial fractions of heart, kidney and liver tissues of rat. Mitochondrial fractions were prepared by the conventional centrifugation method. Ca/sup 2 +/-ATPase activity was determined by measuring the inorganic phosphate liberated during ATP hydrolysis. Toxaphene inhibited Ca/sup 2 +/-ATPase in a concentration dependent manner in all the three tissues. Substrate activation kinetics, with heart, kidney and liver tissue fractions, revealed that toxaphene inhibited Ca/sup 2 +/-ATPase activity non-competetively by decreasing the maximum velocity of the enzyme without affecting the enzyme-substrate affinity. Toxaphene also inhibited mitochondrial /sup 45/Ca/sup 2 +/-uptake in the three selected tissues in a concentration dependent manner. These results indicate that toxaphene is an inhibitor of mitochondrial Ca/sup 2 +/-ATPase and calcium transport in heart, kidney and liver tissues of rat. 19 references, 5 figures.

• Research Organization: Jackson State Univ., MS
• OSTI ID: 5474623
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 36:5; ISSN LIFSA
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
AFFINITY
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARDIOVASCULAR SYSTEM
CATIONS
CELL CONSTITUENTS
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
GLANDS
HEART
HYDROLASES
IN VITRO
INSECTICIDES
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPES
KIDNEYS
LIVER
MAMMALS
MEMBRANE TRANSPORT
MITOCHONDRIA
NUCLEI
ORGANOIDS
ORGANS
PESTICIDES
PHOSPHOHYDROLASES
RADIOISOTOPES
RATS
RODENTS
TOXICITY
UPTAKE
VERTEBRATES