Pulmonary metabolism of dibenz(a,j)acridine: a carcinogenic heterocyclic aromatic. Technical progress report, September 1, 1982-September 30, 1983

Technical Report · Mon Oct 31 04:00:00 EST 1983

OSTI ID:5473894

Warshawsky, D

Dibenz(a,j)acridine (D(a,j)A) is metabolized by rat liver microsomes which results in the formation of seven major metabolites. Under the present conditions of the microsomal assay, 55% of D(a,j)A is metabolized by corn oil induced microsomes in 30 minutes, whereas 80% of D(a,j)A is metabolized by 3MC induced microsomes in 60 minutes. In the presence of the 3MC induced microsomes, a metabolite in fraction 19 is produced very rapidly and disappears linearly over 60 minutes. Of the major metabolites of D(a,j)A produced by microsomal incubations metabolites in fractions 11-12, 13 and 19 are possibly the 5,6-dihydrodiol of D(a,j)A, definitely the 3,4-dihydrodiol of D(a,j)A and a phenol or epoxide respectively. BaP pretreatment of the New Zealand white rabbits doubles the rate of appearance of the metabolites of D(a,j)A in the blood in the IPL. With or without pretreatment, the major metabolites are found in fractions 13 and 19 with 70 to 80% of the activity present in the nonextractable fraction. At the end of the perfusion 50% of the D(a,j)A remains in the IPL following BaP pretreatment as compared to 72% without pretreatment. This increased rate of metabolism due to BaP pretreatment results in the appearance of more conjugated metabolites at the end of the perfusion in the mercaptan fraction.

• Research Organization: Cincinnati Univ., OH (USA). Dept. of Environmental Health
• DOE Contract Number: AC02-82ER60076
• OSTI ID: 5473894
• Report Number(s): DOE/ER/60076-1; ON: DE84002342
• Country of Publication: United States
• Language: English

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ACRIDINES
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ALDEHYDES
ANIMALS
AROMATICS
AZAARENES
AZINES
BENZOPYRENE
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL PREPARATION
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CHROMATOGRAPHY
CONDENSED AROMATICS
DIGESTIVE SYSTEM
DNA ADDUCTS
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EPOXIDES
GLANDS
GLUCURONIC ACID
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXY ACIDS
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LABELLED COMPOUNDS
LABELLING
LIQUID COLUMN CHROMATOGRAPHY
LIVER
LUNGS
MAMMALS
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METABOLIC ACTIVATION
METABOLISM
METABOLITES
MICROSOMES
MIXED-FUNCTION OXIDASES
MUTAGEN SCREENING
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ORGANOIDS
ORGANS
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OXYGENASES
PHENOLS
POLYCYCLIC AROMATIC HYDROCARBONS
PYRIDINES
RABBITS
RADIOCHEMISTRY
RATS
RESPIRATORY SYSTEM
RODENTS
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SPECTRA
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