Xeroderma pigmentosum variants have a slow recovery of DNA synthesis after irradiation with ultraviolet light

Journal Article · Mon Jan 01 04:00:00 EST 1979 · Biochim. Biophys. Acta; (United States)

DOI:https://doi.org/10.1016/0005-2787(79)90193-X· OSTI ID:5473866

Cleaver, J E; Thomas, G H; Park, S D

Human cells (normal and xeroderma pigmentosum variant) irradiated with ultraviolet light and pulse-labelled with (/sup 3/H)thymidine underwent transient decline and recovery of molecular weights of newly synthesized DNA and rates of (/sup 3/H)thymidine incorporation. The ability to synthesize normal-sized DNA recovered more rapidly in both cell types than thymidine incorporation. During recovery cells steadily increased in their ability to replicate normal-sized DNA on damaged templates. The molecular weight versus time curves fitted exponential functions with similar rate constants in normal and heterozygous xeroderma pigmentosum cells, but with a slower rate in two xeroderma pigmentosum variant cell lines. Caffeine added during the post-irradiation period eliminated the recovery of molecular weights in xeroderma pigmentosum variant but not in normal cells. The recovery of the ability to synthesize normal-sized DNA represents a combination of a number of cellular regulatory processes, some of which are constitutive, and one of which is altered in the xeroderma pigmentosum variant such that recovery becomes slow and caffeine sensitive.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 5473866
• Journal Information: Biochim. Biophys. Acta; (United States), Journal Name: Biochim. Biophys. Acta; (United States) Vol. 564; ISSN BBACA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300 -- Cytology
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOSYNTHESIS
CAFFEINE
CELL CULTURES
DATA
DATA FORMS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
INFORMATION
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MOLECULAR WEIGHT
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY
RECOVERY
RESPONSE MODIFYING FACTORS
RIBOSIDES
SYNTHESIS
THYMIDINE
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM
ULTRAVIOLET RADIATION
XANTHINES
XP CELLS
YEARS LIVING RADIOISOTOPES