Continuous measurement of cerebral cortical blood flow by laser-Doppler flowmetry in a rat stroke model

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · Journal of Cerebral Blood Flow and Metabolism; (USA)

DOI:https://doi.org/10.1038/jcbfm.1989.84· OSTI ID:5468225

Dirnagl, U; Kaplan, B; Jacewicz, M; Pulsinelli, W ^[1]

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1. Cornell Univ. Medical College, New York, NY (USA)

Laser-Doppler flowmetry (LDF), a new method allowing instantaneous, continuous, and noninvasive measurements of microcirculatory blood flow in a small tissue sample, was evaluated for its accuracy in monitoring regional cerebral blood flow (rCBF) in the cortical microcirculation after focal cerebral ischemia. Wistar and spontaneously hypertensive rats (SHR, n = 19) were subjected to permanent occlusion of the middle cerebral and common carotid arteries. Absolute rCBF in a tissue sample of the ischemic hemisphere was measured autoradiographically with ({sup 14}C)iodoantipyrine as a tracer and compared to rCBF measured by LDF. Additionally, the percent change in rCBF between baseline and ischemic values was compared for both methods. Absolute rCBF values recorded with LDF correlated poorly (r = 0.54) with ({sup 14}C)iodoantipyrine measurements. In contrast LDF readings expressed as a percentage of ischemic vs. preocclusion readings (relative LDF readings) correlated very well (r = 0.91) with the percent change in (14C)iodoantipyrine measurements. We conclude that LDF does not provide accurate measurements of absolute rCBF values but this method allows accurate measurements of changes in rCBF due to induction of focal cerebral ischemia.

• OSTI ID: 5468225
• Journal Information: Journal of Cerebral Blood Flow and Metabolism; (USA), Journal Name: Journal of Cerebral Blood Flow and Metabolism; (USA) Vol. 9:5; ISSN 0271-678X; ISSN JCBMD
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIPYRETICS
ANTIPYRINE
AUTORADIOGRAPHY
AZOLES
BLOOD FLOW
BODY
BRAIN
CARBON 14 COMPOUNDS
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CEREBRAL CORTEX
CEREBRUM
DISEASES
DRUGS
HETEROCYCLIC COMPOUNDS
ISCHEMIA
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PYRAZOLES
PYRAZOLINES
RATS
RODENTS
VASCULAR DISEASES
VERTEBRATES