The effect of gamma irradiation on injectable human amnion collagen
- Institute of Occupational Medicine, Beijing (China)
The effect of gamma irradiation on the physicochemical properties of injectable human amnion collagen was investigated. Pepsin-extracted human amnion collagen was purified, reconstituted, and irradiated with varying doses of gamma irradiation (0.25 Mrads to 2.5 Mrads). Gamma irradiation had a significant impact on the physical characteristics of the collagen. The neutral solubility of collagen in PBS at 45{degrees}C was decreased from 100% for the nonirradiated control sample to 16% for the 2.5 Mrads irradiated sample. SDS polyacrylamide gel electrophoresis also demonstrated the dose-dependent effect of gamma irradiation on collagen cross-links. Electron microscopic observation revealed that even at low irradiation dose (0.25 Mrads), collagen fibril diameter increased. The average diameter was 50 nm for nonirradiated control fibrils, while 4.4% of the irradiated collagen fibrils had a diameter greater than 100 nm. Irradiated collagen showed little evidence of damage. Well-preserved cross-striations were found in collagen fibrils at all doses of irradiation. Native amnion collagen irradiated with gamma rays demonstrated a slight increase in resistance to collagenase degradation compared with nonirradiated native collagen samples. Increased resistance to collagenase did not correlate with increasing irradiation dose. After 30 min of incubation at 37{degrees}C, both irradiated and nonirradiated collagen was completely digested by collagenase. However, gamma-irradiated collagen did become more sensitive to hydrolysis by trypsin. The higher the irradiation doses used, the greater sensitivity to trypsin was observed. At 0.25 Mrads irradiation only a slight increase was found. No marked differences in amino acid composition were noted among the high dose irradiated, low dose irradiated and control amnion collagen.
- OSTI ID:
- 5467741
- Journal Information:
- Journal of Biomedical Materials Research; (USA), Vol. 23:8; ISSN 0021-9304
- Country of Publication:
- United States
- Language:
- English
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COLLAGEN
CHEMICAL RADIATION EFFECTS
AMINO ACIDS
CHEMICAL PROPERTIES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON MICROSCOPY
ELECTROPHORESIS
GAMMA RADIATION
MAN
PEPTIDE HYDROLASES
PHYSICAL PROPERTIES
PREGNANCY
TRYPSIN
ANIMALS
CARBOXYLIC ACIDS
CHEMISTRY
ELECTROMAGNETIC RADIATION
ENZYMES
HYDROLASES
IONIZING RADIATIONS
MAMMALS
MICROSCOPY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
SCLEROPROTEINS
SERINE PROTEINASES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture