Antipain and radiation effects on oncogenic transformation and sister chromatid exchanges in Syrian hamster embryo and mouse C3H/10T1/2 cells

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/2.12.1229· OSTI ID:5463568

Geard, C R; Rutledge-Freeman, M; Miller, R C; Borek, C

Depending on its time of addition to Syrian hamster embryo or mouse C3H 10T1/2 cells the protease inhibitor antipain (AP) can enhance, or reduce, radiation induced-oncogenic transformations. These opposing influences are not paralleled by changes in sister chromatid exchanges in either cell system. A 24 h treatment with 10 microM AP prior to, and during irradiation, with removal 10 min after irradiation results in greater than a two-fold increase in transformants. Conversely, a 24 h treatment beginning 10 min after irradiation results in about a two-fold decrease in transformants. The utility of AP as an agent for the reduction of tumorigenesis is brought into question, since quite short temporal differences in application can result in near five-fold differences in the frequencies of oncogenic transformations.

• Research Organization: Department of Radiology, Columbia University College of Physician and Surgeons, New York
• OSTI ID: 5463568
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 2:12; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
EMBRYONIC CELLS
ENZYME INHIBITORS
FIBROBLASTS
HAMSTERS
MAMMALS
MUTATIONS
ONCOGENIC TRANSFORMATIONS
RADIATION EFFECTS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RODENTS
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
VERTEBRATES