Expression of human. alpha. -globin and mouse/human hybrid. beta. -globin genes in murine hemopoietic stem cells transduced by recombinant retroviruses

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.11.4349· OSTI ID:5463267

Li, C L; Dwarki, V J; Verma, I M ^[1]

---

1. Salk Institute, San Diego, CA (USA)

Murine cell lines releasing helper-free recombinant retroviruses containing human {alpha}-globin and mouse/human hybrid {beta}-globin genes were generated. The expression of the hybrid {beta}-globin gene but not the human {alpha}-globin gene was regulated appropriately in infected mouse erythroid leukemia (MEL) cells. Murine bone marrow cells were infected by coculture with virus-producing cells and transplanted into lethally irradiated syngeneic recipients. Greater than 90% of the spleen colonies (12-15 days), which are derived from hemopoietic multipotential stem cells, showed proviral integration. Various levels of expression of the transduced globin genes were detected in all of the provirus-positive spleen colonies. Proviral sequences and transcripts from the transduced globin genes could also be detected in a few long-term reconstituted recipients in an observation period of 10 months after transplantation.

• OSTI ID: 5463267
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:11; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BODY
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
DNA SEQUENCING
GENE RECOMBINATION
GENE REGULATION
GLOBINS
HEMATOPOIETIC SYSTEM
MAMMALS
MICE
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
STEM CELLS
STRUCTURAL CHEMICAL ANALYSIS
THERAPY
VERTEBRATES
VIRUSES