Interaction of leukotriene C4 and Chinese hamster lung fibroblasts (V79A03 cells). 1. Characterization of binding

Technical Report · Mon Oct 01 04:00:00 EDT 1990

OSTI ID:5459970

Fitz, T A; Contois, D F; Liu, Y X; Watt, D S; Walden, T L

A novel, specific, and potent biological action of leukotriene C4 (LTC4) was demonstrated in the Chinese Hamster lung fibroblast cell line V79A03 (V79 cells), namely the conferment of protection against subsequent irradiation. Consequently, studies were conducted to determine whether LTC4-conferred radioprotection could be attributed to a receptor-mediated phenomenon. Specific binding sites for leukotriene C4 (LTC4) were identified and characterized using intact V79 cells incubated at 4 C in the presence of serine-borate, during which time conversion of LTC4 to LTD4 or LTE4 was undetectable. Binding was maximal in a broad region between pH 6.2 and 8.8. Ca2+, Mg2+, and Na+ were not required for binding, and binding was not altered by GTP, ATP, cAMP, by leukotrienes B4, D4, or E4, or by the leukotriene end point antagonists LY 171883, FPL 55712, or Revlon 5901-5.

• Research Organization: Armed Forces Radiobiology Research Inst., Bethesda, MD (United States)
• OSTI ID: 5459970
• Report Number(s): AD-A-235709/3/XAB; AFRRI-SR--91-4
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CONNECTIVE TISSUE CELLS
FIBROBLASTS
HAMSTERS
IRRADIATION
KINETICS
LUNGS
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PROSTAGLANDINS
PROTEINS
RADIATION PROTECTION
RADIOSENSITIVITY EFFECTS
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
VERTEBRATES