Demonstration of NK cell-mediated lysis of varicella-zoster virus (VZV)-infected cells: characterization of the effector cells

Journal Article · Sun Jun 01 00:00:00 EDT 1986 · J. Immunol.; (United States)

OSTI ID:5459090

Tilden, A B; Cauda, R; Grossi, C E; Balch, C M; Lakeman, A D; Whitley, R J

Infection with varicella-zoster virus (VZV) rendered RAJI cells more susceptible to lysis by non-adherent blood lymphocytes. At an effector to target ratio of 80:1 the mean percentage of /sup 51/Cr release of VZV-infected RAJI cells was 41 +/- 12%, whereas that of uninfected RAJI cells was 15 +/- 6%. The increased susceptibility to lysis was associated with increased effector to target conjugate formation in immunofluorescence binding assays. The effector cells cytotoxic for VZV-infected RAJI cells were predominantly Leu-11a/sup +/ Leu-4/sup -/ granular lymphocytes as demonstrated by fluorescence-activated cell sorting. The effector cell active against VZV-infected RAJI cells appeared similar to those active against herpes simplex virus (HSV)-infected cells, because in cold target competition experiments the lysis of /sup 51/Cr-labeled VZV-infected RAJI cells was efficiently inhibited by either unlabeled VZV-infected RAJI cells (mean 71% inhibition, 2:1 ratio unlabeled to labeled target) or HSV-infected RAJI cells (mean 69% inhibition) but not by uninfected RAJI cells (mean 10% inhibition). In contrast, competition experiments revealed donor heterogeneity in the overlap between effector cells for VZV- or HSV-infected RAJI vs K-562 cells.

• Research Organization: Univ. of Alabama, Birmingham
• OSTI ID: 5459090
• Journal Information: J. Immunol.; (United States), Vol. 136:11
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
LYMPHOCYTES
IMMUNE REACTIONS
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551001* - Physiological Systems- Tracer Techniques