Detection of radicals produced in vivo during inhalation exposure to ozone: Use of various spin traps
Ozone is known to induce lipid peroxidation of lung tissue, although no direct evidence of free radical formation has been reported. The study used the electron paramagnetic resonance (EPR) spin-trapping technique to search for free radicals produced in vivo by ozone exposure. The spin trap alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone (4-POBN) was administered i.p. to male Sprague-Dawley rats. The rats were then exposed for 2 hrs to either 0, 0.5, 1.0, 1.5 or 2.0 ppm ozone with 8% CO{sub 2} to increase their respiratory rate. A 6-line 4-POBN/radical spin adduct signal a{sup N}=15.02 G and a{sup H}beta-3.27 G was detected by EPR spectroscopy in lipid extracts from lungs of rats treated with 4-POBN and then exposed to ozone. Only a very weak signal was observed in the corresponding solution from rats exposed to 0 ppm ozone (air with CO{sub 2} only). The concentration of the radical adduct increased as a function of ozone concentration. After administration of 4-POBN, rats were exposed for either 0.5, 1.0, 2.0 or 4.0 hrs to either 0 or 2.0 ppm ozone (with CO{sub 2}). These results demonstrate that ozone induces the production of free radicals in rat lungs during inhalation exposure and that radical production may be involved in the induction of lung edema by ozone.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC (United States). Health Effects Research Lab.
- OSTI ID:
- 5458741
- Report Number(s):
- PB-91-196113/XAB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOSYNTHESIS
BODY
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
EDEMA
ELECTRON SPIN RESONANCE
INHALATION
INTAKE
LIPIDS
LUNGS
MAGNETIC RESONANCE
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
OZONE
PATHOLOGICAL CHANGES
RADICALS
RATS
RESONANCE
RESPIRATORY SYSTEM
RODENTS
SYMPTOMS
SYNTHESIS
TOXICITY
VERTEBRATES