Search for oxygen-centered free radicals in the lipoxygenase/linoleic acid system
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
Studies of the oxygenation of linoleic acid by soybean lipoxygenase utilizing electron spin resonance spectroscopy and oxygen uptake have been undertaken. The spin trap, alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone (4-POBN) was included in the lipoxygenase system to capture short-lived free radicals. Correlation of radical adduct formation rates with oxygen uptake studies indicated that the major portion of radical adduct formation occurred when the system was nearly anaerobic. Incubations containing (/sup 17/O)oxygen with nuclear spin of 5/2 did not have additional ESR lines as would be expected if an oxygen-centered 4-POBN-lipid peroxyl radical adduct were formed indicating that the trapped radical must be reassigned as a carbon-centered species. To establish the presence of (/sup 17/O/sub 2/)oxygen in our incubations, a portion of the gas from the lipoxygenase/linoleate experiments was used to prepare the 4-POBN-superoxide radical adduct utilizing a superoxide producing microsomal/paraquat/NADPH system.
- Research Organization:
- National Institute of Environmental Health Sciences, Research Triangle Park, NC
- OSTI ID:
- 6935275
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 2; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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·
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· Biochem. Biophys. Res. Commun.; (United States)
·
OSTI ID:6846004
Related Subjects
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
CARBOXYLIC ACIDS
CATALYSIS
CHALCOGENIDES
ELECTRON SPIN RESONANCE
ELEMENTS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
FOOD
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LINOLEIC ACID
MAGNETIC RESONANCE
METABOLISM
MONOCARBOXYLIC ACIDS
NITROGEN COMPOUNDS
NITROGEN OXIDES
NONMETALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN
OXYGEN 17
OXYGEN COMPOUNDS
OXYGEN ISOTOPES
OXYGENASES
PLANTS
RADICALS
RESONANCE
SOYBEANS
STABLE ISOTOPES
SUPEROXIDE RADICALS
TRACER TECHNIQUES
VEGETABLES
59 BASIC BIOLOGICAL SCIENCES
CARBOXYLIC ACIDS
CATALYSIS
CHALCOGENIDES
ELECTRON SPIN RESONANCE
ELEMENTS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
FOOD
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LINOLEIC ACID
MAGNETIC RESONANCE
METABOLISM
MONOCARBOXYLIC ACIDS
NITROGEN COMPOUNDS
NITROGEN OXIDES
NONMETALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN
OXYGEN 17
OXYGEN COMPOUNDS
OXYGEN ISOTOPES
OXYGENASES
PLANTS
RADICALS
RESONANCE
SOYBEANS
STABLE ISOTOPES
SUPEROXIDE RADICALS
TRACER TECHNIQUES
VEGETABLES