Characteristics of arterial Na-K-ATPase in WKY and SHR
Segments of carotid and tail arteries and thoracic aorta from 12-week old male WKY and SHR were used to study Na-pump activity by /sup 86/Rb-uptake method. Contiguous segments were used for the determination of cell Na content by ion substitution. The ouabain-dependence of maximum Na-pump activity was determined. The EC/sub 50/ value of ouabain (approximately 0.1 mM) was not significantly different for WKY and SHR arteries or at any of the three arterial sites. The maximum value of /sup 86/Rb-uptake was significantly larger of all SHR arteries compared to WKY. No significant differences in ouabain-insensitive /sup 86/Rb-uptake was found. No significant differences exist in estimates of cell Na in arteries from the two groups. Segments of arteries were stored in K-free PSS overnight at 2/sup 0/C, and allowed to recover in normal PSS at 37/sup 0/C for varying periods of time. Ouabain-sensitive /sup 86/Rb-uptake and estimates of cell Na were determined during recovery from cold storage. There were no significant differences in the EC/sub 50/ value of Na, which produced a half-maximal ouabain-sensitive /sup 86/Rb-uptake between WKY and SHR. The only difference observed in these studies was a larger turnover rate of the Na-pump in arteries from the SHR.
- Research Organization:
- Univ. of Pennsylvania, Philadelphia
- OSTI ID:
- 5453963
- Report Number(s):
- CONF-8604222-; TRN: 86-026568
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ATP-ASE
ENZYME ACTIVITY
SODIUM
MEMBRANE TRANSPORT
ARTERIES
COMPARATIVE EVALUATIONS
OUABAIN
POTASSIUM
RATS
RUBIDIUM 86
TRACER TECHNIQUES
ACID ANHYDRASES
ALKALI METAL ISOTOPES
ALKALI METALS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
ENZYMES
GLYCOSIDES
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHOHYDROLASES
RADIOISOTOPES
RODENTS
RUBIDIUM ISOTOPES
STROPHANTHINS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques