Reciprocal expression of human ETS1 and ETS2 genes during T-cell activation: Regulatory role for the protooncogene ETS1

Journal Article · Tue May 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.10.3723· OSTI ID:5451529

Bhat, N K ^[1]; Thompson, C G; Lindsten, T ^[2]; June, C H ^[3]; Fujiwara, Shigeyoshi; Koizumi, Shigeki; Fisher, R J; Papas, T S ^[4]

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1. National Cancer institute, Frederick, MD (USA) Program Resources, Inc., Frederick, MD (USA)
2. Univ. of Michigan, Ann Arbor (USA)
3. Naval Medical Research Institute, Bethesda, MD (USA)
4. National Cancer Institute, Frederick, MD (USA)

The expression of the protooncogenes ETS1 and ETS2 has been studied in purified human T cells activated either by cross-linking of the T-cell receptor-CD3 complex on their cell surface or by direct stimulation with phorbol esters and ionomycin. The results show that resting T cells express high levels of ETS1 mRNA and protein, while expression of ETS2 is undetectable. Upon T-cell activation, ETS2 mRNA and proteins are induced, while ETS1 gene expression decreases to very low levels. Late after stimulation, ETS1 mRNA is reinduced and maintained at a high level, while ETS2 gene expression decreases to undetectable levels. Therefore, it appears that in human T cells, ETS2 gene products are associated with cellular activation and proliferation, while ETS1 gene products are preferentially expressed in a quiescent state.

• OSTI ID: 5451529
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:10; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CARCINOGENS
CELL PROLIFERATION
CHEMICAL ACTIVATION
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
DRUGS
ESTERS
EVEN-ODD NUCLEI
GENE REGULATION
GENES
HYBRIDIZATION
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MESSENGER-RNA
METHIONINE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYMERIZATION
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
RNA
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
VERTEBRATES