Apolipoprotein J: Structure and tissue distribution

Journal Article · Tue Jun 05 00:00:00 EDT 1990 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00474a025· OSTI ID:5451329

de Silva, H V; Harmony, J A.K.; Stuart, W D; Gil, C M; Robbins, J ^[1]

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1. Univ. of Cincinnati College of Medicine, OH (USA)

The primary structure of apolipoprotein J (apoJ) was deduced by the combined strategies of protein sequencing and cDNA cloning and sequencing. ApoJ, an apolipoprotein associated with discrete subclasses of high-density lipoproteins, is encoded by a single gene in both the human and mouse genomes. ApoJ is synthesized as a 427 amino acid polypeptide that is posttranslationally cleaved at an internal bond between Arg-205 and Ser-206. The subunits of apoJ are designated apoJ{alpha}, corresponding to residues 1-205, and apoJ{beta}, corresponding to residues 206-427. The subunits are associated through disulfide bonds. Analysis of the primary structure of apoJ predicts the existence of amphiphilic helices, which may account for the association of apoJ with lipoproteins, and heparin-binding motifs in both subunits. ApoJ appears to be the human analogue of a rat protein present in high concentrations in the testis, sulfated glycoprotein 2. ApoJ mRNA (1.9 kb) is expressed in all but one tissue examined. The mRNA is present in relatively high levels in brain, ovary, testis, and liver, is less abundant in heart, spleen, lung, and breast, and is absent in T-lymphocytes. ApoJ is unique among previously characterized human apolipoproteins in its structure and tissue distribution.

• OSTI ID: 5451329
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 29:22; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
APOLIPOPROTEINS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
DNA
DNA SEQUENCING
FEMALE GENITALS
GLANDS
GLYCOPROTEINS
GONADS
HEART
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
LIVER
LUNGS
MALE GENITALS
MAMMALS
MAMMARY GLANDS
MAN
MESSENGER-RNA
MOLECULAR STRUCTURE
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OVARIES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RATS
RECOMBINANT DNA
RESPIRATORY SYSTEM
RNA
RODENTS
SPLEEN
STRUCTURAL CHEMICAL ANALYSIS
TESTES
TISSUE DISTRIBUTION
VERTEBRATES