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Relationship between induction of ethoxyresorufin-O-deethylase (FROD) and insulin binding in tissues of rat placenta

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5451310
; ; ;

The authors have reported that treatment of pregnant rats with ..beta..-naphthoflavone (..beta.. NF) resulted in differential induction of EROD in placental tissues. This study further characterized the induction of EROD in placental tissues by 3-methylcholanthrene (3MC), and investigated whether there is a relationship between the induction of EROD and insulin binding to the placental tissues. ..beta.. NF (15 mg/kg) or 3-MC (30 mg/kg) was administered i.p. on gestation day 14. On day 15, placentae were dissected into basal zone (BZ) and labyrinth (LA) tissues. EROD activity was measured by fluorimetric assay in homogenates of BZ or LA. Plasma membranes of BZ and LA were prepared and assayed for insulin binding using (/sup 125/I)-insulin. In summary, treatment with ..beta.. NF as well as 3-MC differentially induced EROD in LA compared to BZ tissue, while neither treatment affected the binding of insulin to BZ and LA membranes.

Research Organization:
Univ. of Florida, Gainesville
OSTI ID:
5451310
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
FETAL MEMBRANES
GENE REGULATION
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
OXIDOREDUCTASES
PEPTIDE HORMONES
PLACENTA
RADIOASSAY
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
VERTEBRATES