Alteration in morphology of Chinese hamster ovary cells by Ni/sub 3/S/sub 2/ and dibutyryl cAMP

Journal Article · Sun Jan 01 04:00:00 EST 1978 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(78)90263-6· OSTI ID:5450869

Costa, M

Rounded Chinese hamster ovary cells (CHO) (axial ratio, 1:1) elongated (axial ratio, > 1:3) when exposed to Ni/sub 3/S/sub 2/. Other metal compounds which were tested had only minor effects on CHO morphology over a wide range of concentrations. These cells were elongated following exposure to Ni/sub 3/S/sub 2/ at concentrations which did not affect cell proliferation as judged by cell doubling and colony formation. The elongation occurred when agents that elevate intracellular cAMP concentrations were added to cultures. The cells treated with either dibutyryl cAMP or Ni/sub 3/S/sub 2/ had very similar fibroblastic morphology. Upon removal of media containing dibutyryl cAMP, elongated CHO cells reverted to control morphology within 4 h. In contrast, the cultures previously treated with Ni/sub 3/S/sub 2/ did not rapidily revert to normal morphology. Nickel subsulfide activated cAMP-dependent protein kinase within 3 h after its addition to cultures while NiS or NiCl/sub 2/ did not significantly activate protein kinase. Dibutyryl cAMP addition to CHO cultures completely activated this enzyme. High doses of actinomycin D, or cycloheximide did not affect the dibutyryl cAMP-induced elongation of CHO cells while addition of colcemid did. All three of these agents inhibited the Ni/sub 3/S/sub 2/ elongation of CHO cells. These data demonstrate that Ni/sub 3/S/sub 2/ induced morphological changes in CHO cells are similar to the morphological changes mediated by cAMP.

• Research Organization: Univ. of Connecticut, Storrs
• OSTI ID: 5450869
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 44; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTINOMYCIN
AMP
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
ANTIMITOTIC DRUGS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL RECOVERY
CELL CULTURES
CELL PROLIFERATION
COLONY FORMATION
CYCLOHEXIMIDE
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELONGATION
ENZYMES
FUNGICIDES
HAMSTERS
KINETICS
MAMMALS
MORPHOLOGICAL CHANGES
NICKEL COMPOUNDS
NUCLEOTIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PESTICIDES
PHOSPHOTRANSFERASES
REACTION KINETICS
RECOVERY
RESPONSE MODIFYING FACTORS
RODENTS
SULFITES
SULFUR COMPOUNDS
TOXICITY
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES