Chloride dependence of intracellular pH in frog skin: a /sup 31/P NMR study

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5449894

Peterson-Yantorno, K; Civan, M M

Single frog skins from Northern Variety Rana pipiens were analyzed by /sup 31/P NMR spectroscopy during superfusion alternately with control and experimental Ringer's solutions, permitting each preparation to serve as its own control. The spectral positions of intracellular inorganic phosphate and extracellular methylphosphonate permitted continuous monitoring of intracellular (pH/sub c/) and extracellular (pH/sub 0/) pH, respectively. Acute and steady state measurements suggested that pH/sub c/ is well regulated at about 7.29 +- 0.05 over an external pH range of about 7.25-7.65. Below this range, pH/sub c/ decreased markedly when the external pH was reduced with nonvolatile acid. In the presence of 2.5 mM HCO/sub 3//sup -/ and 1% CO/sub 2/, total substitution of external Cl/sup -/ by gluconate reversibly increased pH/sub c/ by 0.34 +- 0.05 U (mean +- SE). Replacing external Cl/sup -/ by SO/sup 2 -//sub 4/ increased pH/sub c/ by 0.12 +- 0.01 in the presence of external HCO/sup -//sub 3/, but only by 0.05 +- 0.02 in its absence. SITS (1 mM) at a pH/sub 0/ of 6.95 +- 0.05 did not significantly alter pH/sub c/, but entirely prevented the steady state alkalinization characteristically induced by gluconate substitution for external Cl/sup -/. The results document that: (1) intracellular pH is maintained relatively constant when the external pH is varied over the physiologic range by adding fixed acid or base, and (2) this regulation is (at least in part) a reflection of Cl/HCO/sub 3/ antiport activity.

• Research Organization: Univ. of Pennsylvania School of Medicine, Philadelphia
• OSTI ID: 5449894
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550600* -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACID CARBONATES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BIOCHEMICAL REACTION KINETICS
BODY
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
CHLORIDES
CHLORINE COMPOUNDS
FROGS
GLUCONIC ACID
HALIDES
HALOGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PH VALUE
PHOSPHATES
PHOSPHORUS 31
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
REACTION KINETICS
RESONANCE
SKIN
SPECTRA
STABLE ISOTOPES
VERTEBRATES