Microelectrode study of intracellular pH in frog skin: dependence on serosal Cl/sup -/

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5320469

Duffey, M E; Kelepouris, E; Peterson-Yantorno, K; Civan, M M

Replacing external Cl/sup -/ reduces Na/sup +/ transport across frog skin, but the sidedness and mechanisms have been unclear. We have monitored current (l/sub T/), resistance (R/sub T/) and basolateral membrane potential, both with reference micropipettes (psi/sup sc/) and pH-selective microelectrodes (E/sub H//sup sc/), in short-circuited epithelial sheets isolated from frog skins; removal of the dermis facilitates basolateral exchange. Intracellular pH was 7.25 +- 0.03 (mean +- SE) when the pH of the external Cl/sup -/ Ringer's solution was 7.60 +- 0.01, in reasonable agreement with estimates from /sup 31/P and /sup 19/F NMR analyses. Complete mucosal replacement of Cl/sup -/ by gluconate had variable effects on l/sub T/ and R/sub T/. However, serosal Cl/sup -/ substitution uniformly increased R/sub T/ and markedly decreased l/sub T/, absolute value phi/sup sc/ and absolute value E/sub A//sup sc/. The membrane depolarization was usually preceded by a small hyperpolarization (0.5-3.5 mV). The serosal Cl/sup -/ replacement also produced an intracellular alkalinization of 0.4 +- 0.1 U. These data suggest that: (1) serosal Cl/sup -/ substitution alkalinizes the cells by either enhancing HCO/sup -/ entry or blocking HCO/sup -/ loss through a basolateral Cl/HCO antiport, and (2) the fall in absolute value phi/sup sc/ and l/sub T/ may partly reflect inhibition of apical Na/sup +/ entry, produced indirectly by membrane depolarization resulting from altered basolateral ionic conductances.

• Research Organization: Univ. of Pennsylvania School of Medicine, Philadelphia
• OSTI ID: 5320469
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

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550600* -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
AMPHIBIANS
ANIMAL TISSUES
ANIMALS
AQUATIC ORGANISMS
BIOCHEMICAL REACTION KINETICS
BIOELECTRICITY
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHLORINE COMPOUNDS
DEPOLARIZATION
ELECTRICITY
EPITHELIUM
FLUORINE 19
FLUORINE ISOTOPES
FROGS
GLUCONIC ACID
HALOGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
MEMBRANE TRANSPORT
MEMBRANES
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PH VALUE
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
REACTION KINETICS
RESONANCE
SKIN
SODIUM COMPOUNDS
SPECTRA
STABLE ISOTOPES
TISSUES
VERTEBRATES