Influence of CO sub 2 on electrophysiology and ionic permeability of the basolateral membrane of frog skin

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1984

OSTI ID:6974571

Stoddard, J S

When short-circuited epithelia of frog skin bathed in an alkaline Ringer solution equilibrated with room air, are exposed to a Ringer solution equilibrated with 5% CO{sub 2}, inhibition of transepithelial Na{sup +} transport is observed accompanied by a marked depolarization of the basolateral membrane voltage as measured with intracellular microelectrodes. To study further the mechanisms involved, basolateral membrane influxes and effluxes of {sup 24}Na, {sup 42}K, and {sup 36}Cl were measured in control and CO{sub 2}-treated isolated epithelia. In control epithelia, studies of the bidirectional {sup 24}Na fluxes confirmed the existence of an important basolateral membrane permeability to Na{sup +}. In control epithelia, the apical membranes of the cells were found to be virtually impermeable to Cl{sup {minus}}, while basolateral membranes were highly permeable to Cl{sup {minus}}. Although CO{sub 2} caused a partial inhibition of pump activity as assessed from decreases of pump-mediated Na{sup +} efflux and K{sup +} influx, CO{sub 2} caused little or no change of the leak influx of Na{sup +} or K{sup +}. K{sup +} efflux was increased markedly with CO{sub 2} resulting in a net loss of K{sup +} from the cells. Cl{sup {minus}} influx was increased and Cl{sup {minus}} efflux was decreased by CO{sub 2} leading to a net influx of Cl{sup {minus}}. Analysis of the data according to criteria involving changes of flux, ionic equilibrium potentials, mass and charge balance restrictions indicated that the principle changes involve a transient decrease in electrical conductance to K{sup +} with a concurrent increase in electrical conductance to HCO{sub 3}{sup {minus}}(OH{sup {minus}} or H{sup +}) of the basolateral membranes of the cells.

• Research Organization: Illinois Univ., Urbana, IL (USA)
• OSTI ID: 6974571
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL ISOTOPES
AMPHIBIANS
ANIMAL TISSUES
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOELECTRICITY
BIOLOGICAL EFFECTS
BODY
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CELL CONSTITUENTS
CELL MEMBRANES
CHALCOGENIDES
CHLORINE 36
CHLORINE ISOTOPES
ELECTRICITY
ELECTRON CAPTURE RADIOISOTOPES
EPITHELIUM
FROGS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIGHT NUCLEI
MEMBRANE TRANSPORT
MEMBRANES
NUCLEI
ODD-ODD NUCLEI
ORGANS
OXIDES
OXYGEN COMPOUNDS
PERMEABILITY
PHYSIOLOGY
POTASSIUM 42
POTASSIUM ISOTOPES
RADIOISOTOPES
SECONDS LIVING RADIOISOTOPES
SKIN
SODIUM 24
SODIUM ISOTOPES
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES