Reduction in DNA repair capacity following differentiation of murine proadipocytes

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90319-9· OSTI ID:5448808

Tofilon, P J; Meyn, R E ^[1]

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1. Univ. of Texas M.D. Anderson Hospital and Tumor Institute, Houston (USA)

It has been suggested that terminally differentiated mammalian cells have a decreased DNA repair capacity, compared with proliferating stem cells. To investigate this hypothesis, we have examined {gamma}-ray-induced DNA strand breaks and their repair in the murine proadipocyte stem cell line 3T3-T. By exposure to human plasma, 3T3-T cells can be induced to undergo nonterminal and then terminal differentiation. DNA strand breaks were evaluated using the technique of alkaline elution. No difference was detected among stem, nonterminally differentiated, and terminally differentiated cells in the initial levels of radiation-induced DNA strand breaks. Each of the strand break dose responses increased as a linear function of {gamma}-ray dose. The strand breaks induced by 4 Gy rejoined following biphasic kinetics for each cell type. At each time point examined after irradiation, however, the percentage of strand breaks that had not rejoined in terminally differentiated cells was three to six times greater than in stem cells. The rate of strand break rejoining in nonterminally differentiated cells was of an intermediate value between that of the stem and of the terminally differentiated cells. These results indicate that, at least for 3T3-T cells, differentiated cells have a reduced capacity for DNA repair.

• OSTI ID: 5448808
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 174:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADIPOSE TISSUE
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
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BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
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CELL CULTURES
CELL DIFFERENTIATION
CESIUM 137
CESIUM ISOTOPES
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DNA REPAIR
ELECTROMAGNETIC RADIATION
FAT CELLS
GAMMA RADIATION
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
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MICE
NUCLEI
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ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SOMATIC CELLS
STRAND BREAKS
THYMIDINE
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES