In vivo stem cell function of interleukin-3-induced blast cells
- Department of Medicine, Jichi Medical School, Tochigi-ken (Japan)
The treatment of mice with high doses of 5-fluorouracil (5-FU) results in an enrichment of primitive hematopoietic progenitors. Using this procedure, the authors obtained a new class of murine hematopoietic colonies that had very high secondary plating efficiencies in vitro and could differentiate into not only myeloid cells but also into lymphoid lineage cells. The phenotypes of interleukin-3 (IL-3) induced blast colony cells were Thy-1-positive and lineage-marker-negative. They examined whether these blast colony cells contained primitive hematopoietic stem cells in vivo and could reconstitute hematopoietic tissues in lethally irradiated mice. Blast colony cells could generate macroscopic visible spleen colonies on days 8 and 12, and 5 {times} 10(3) blast cells were sufficient to protect them from lethally irradiation. It was shown that 6 or 8 weeks after transplantation of 5 {times} 10(3) blast cells, donor male cells were detected in the spleen and thymus of the female recipients but not in the bone marrow by Southern blot analysis using Y-encoded DNA probe. After 10 weeks, bone marrow cells were partially repopulated from donor cells. In a congenic mouse system, donor-derived cells (Ly5.2) were detected in the thymus and spleen 6 weeks after transplantation. Fluorescence-activated cell sorter analyses showed that B cells and macrophages developed from donor cells in the spleen. In the thymus, donor-derived cells were found in CD4, CD8 double-positive, single-positive, and double-negative populations. Reconstitution of bone marrow was delayed and myeloid and lymphoid cells were detected 10 weeks after transplantation. These results indicate that IL-3-induced blast cells contain the primitive hematopoietic stem cells capable of reconstituting hematopoietic organs in lethally irradiated mice.
- OSTI ID:
- 5448356
- Journal Information:
- Blood (Journal of Hematology); (United States), Journal Name: Blood (Journal of Hematology); (United States) Vol. 78:2; ISSN 0006-4971; ISSN BLOOA
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CHIMERAS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DNA
GROWTH FACTORS
HEMATOPOIETIC SYSTEM
KINETICS
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOCYTES
LYMPHOKINES
MACROPHAGES
MAMMALS
MATERIALS
MICE
MITOGENS
MOSAICISM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PHENOTYPE
PROTEINS
RADIATION CHIMERAS
REACTION KINETICS
RODENTS
SOMATIC CELLS
SPLEEN
STEM CELLS
THYMUS
TISSUES
TRANSPLANTS
VERTEBRATES