Fetal guinea pig brain 15-hydroxyprostaglandin dehydrogenase: Ontogeny and effect of ethanol

Journal Article · Fri Mar 01 04:00:00 EST 1991 · Alcohol; (United States)

DOI:https://doi.org/10.1016/0741-8329(91)91316-T· OSTI ID:5447384

Treissman, D; Brien, J F ^[1]

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1. Department of Pharmacology and Toxicology, Faculty of Medicine, Queen's University, Kingston, Ontario (Canada)

The objectives of this study were to determine the ontogeny of 15-hydroxyprostaglandin dehydrogenase (15-OH-PGDH) activity in the brain of the fetal guinea pig and to test the hypothesis that acute in vitro ethanol exposure produces concentration-dependent inhibition of fetal brain 15-OH-PGDH activity. Enzyme activity was determined in vitro by measuring the rate of oxidation of PGE2 to 15-keto-PGE2 using an optimized radiometric procedure. The study was conducted utilizing the whole brain of the fetal guinea pig at mean gestational ages of 34, 43 and 62 days (term, about 66 days) and the brain stem (pons and medulla) of the fetal guinea pig at mean gestational ages of 43 and 62 days. The direct effect of acute in vitro exposure to ethanol was assessed by incubating 15-OH-PGDH with ethanol in the concentration range of 10 to 80 mM. 15-OH-PGDH was measurable in the whole brain and brain stem, and the enzyme activity was similar for the gestational ages examined. There was no significant ethanol-induced inhibition of 15-OH-PGDH activity in the whole brain or brain stem. The data demonstrate that the whole brain and brain stem of the fetal guinea pig have the capacity to metabolize PGE2 to 15-keto-PGE2, an inactive metabolite, during the second half of gestation. The data apparently are not consistent with the hypothesis that acute in vitro exposure to ethanol directly inhibits 15-OH-PGDH activity in fetal brain.

• OSTI ID: 5447384
• Journal Information: Alcohol; (United States), Journal Name: Alcohol; (United States) Vol. 8:2; ISSN 0741-8329; ISSN ALCOE
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ALCOHOLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
ENZYME ACTIVITY
ENZYMES
ETHANOL
FETUSES
GUINEA PIGS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
MAMMALS
METABOLISM
NERVOUS SYSTEM
ONTOGENESIS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PROSTAGLANDINS
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES