Structure, expression, and molecular mapping of a divergent member of the class I HLA gene family

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.12.4224· OSTI ID:5445599

Srivastava, R; Chorney, M J; Lawrance, S K; Pan, J; Smith, Z; Smith, C L; Weissman, S M

A class I gene distinct from HLA-A, -B, or -C was identified in a cosmid clone and transfected into mouse L cells. The gene, placed adjacent to the polyoma enhancer, produced a full-length class I mRNA and high levels of a 43-kDa protein in the cytoplasm. The surface expression of the gene product required its association with human ..beta../sub 2/-microglobulin. The protein was recognized by a xenoantiserum raised against a mixture of human B- and T-cell lines. The product was also serologically reactive the HLA framework monoclonal antibodies. The complete nucleotide sequence of the gene was determined and a specific oligonucleotide probe was synthesized. This probe was used to identify a full-length mRNA transcript in a B-lymphoblastoid cell line (JY). The gene was mapped within a 190-kilobase Not I restriction fragment located in the telomeric portion of the human major histocompatibility complex. Distinct features of the gene include (i) the structure of the promoter, (ii) the position of the translation initiation site, (iii) a frameshift mutation at the carboxyl terminus, (iv) the insertion of an Alu repeat element in the eighth exon, (v) divergence in the derived amino acid sequence, and (vi) the lack of expression of the gene in some cells.

• Research Organization: Yale Univ. School of Medicine, New Haven, CT
• OSTI ID: 5445599
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:12; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANIMALS
ANTIGENS
AUTORADIOGRAPHY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMES
CLONING
CONNECTIVE TISSUE CELLS
DNA
DNA SEQUENCING
DNA-CLONING
ELECTROPHORESIS
GENES
GENETIC MAPPING
HETEROCHROMOSOMES
IMMUNITY
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MAPPING
MATERIALS
MICE
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TUMOR CELLS
VERTEBRATES