Portal copper transport in rats by albumin

Journal Article · Sun Mar 01 04:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:5442331

Gordon, D T; Leinart, A S; Cousins, R J

The distribution of newly absorbed copper among serum proteins obtained from the portal circulation of rats was examined by conventional and high-performance gel filtration chromatography, affinity chromatography, and Western blotting. Within 10-30 min after being administered by gavage or directly into the intestine, /sup 67/Cu and /sup 64/Cu, respectively, were recovered in the albumin fraction. By 8 h after administration of the radionuclides, virtually all of the radioactivity was found with ceruloplasmin. Affigel blue fractionation and subsequent Superose-6 chromatography further demonstrated that all of the copper in the albumin-containing fractions was in fact bound to this protein rather than high molecular weight moieties. Vascular perfusion of the isolated rat intestine, where /sup 64/Cu was infused into the lumen, showed that newly absorbed /sup 64/Cu in the vascular perfusate collected from the cannulated portal vein was associated with albumin. Uptake of radioactivity by isolated rat liver parenchymal cells from medium containing rat serum with /sup 67/Cu bound to albumin was demonstrated. In vitro binding of /sup 64/Cu to serum proteins that were transferred to nitrocellulose by Western blotting techniques showed that albumin is essentially the only protein that binds appreciable amounts of copper. The data suggest that albumin is the plasma protein that is responsible for the initial transport of copper after absorption.

• Research Organization: Univ. of Florida, Gainesville
• OSTI ID: 5442331
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:3; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ALBUMINS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BLOOD SERUM
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CERULOPLASMIN
CHEMISTRY
CHROMATOGRAPHY
COMPLEXES
COPPER 64
COPPER 67
COPPER COMPLEXES
COPPER ISOTOPES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
GASTROINTESTINAL TRACT
GEL PERMEATION CHROMATOGRAPHY
GLANDS
GLOBULINS
GLOBULINS-ALPHA
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
INTESTINAL ABSORPTION
ISOTOPES
LIVER
MAMMALS
METALLOPROTEINS
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORAL ADMINISTRATION
ORGANIC COMPOUNDS
ORGANS
PHYSIOLOGY
PORTAL SYSTEM
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SEPARATION PROCESSES
TRACE AMOUNTS
TRANSITION ELEMENT COMPLEXES
UPTAKE
VEINS
VERTEBRATES