Lysis of cells infected with typhus group rickettsiae by a human cytotoxic T cell clone

Journal Article · Tue Dec 15 04:00:00 EST 1987 · J. Immunol.; (United States)

OSTI ID:5441828

Carl, M; Robbins, F; Hartzman, R J; Dasch, G A

Cytolytic human T cells clones generated in response to the intracellular bacterium Rickettsia typhi were characterized. Growing clones were tested for their ability to proliferate specifically in response to antigens derived from typhus group rickettsiae or to lyse targets infected with R. typhi or Rickettsia prowazekii, as measured by /sup 51/Cr-release from target cells. Two clones were able to lyse targets infected with typhus group rickettsiae. One of these clones was more fully characterized because of its rapid growth characteristics. This cytolytic clone was capable of lysing an autologous infected target as well as a target matched for class I and II histocompatibility leukocyte antigens (HLA). It was not capable, however, of lysing either a target mismatched for both class I and II HLA or a target partially matched for class I HLA. In addition, the clone exhibited specificity in that it was able to lyse an autologous target infected with typhus group rickettsiae, but did not lyse an autologous target infected with an antigenically distinct rickettsial species, Rickettsia tsutsugamushi. These results demonstrate, for the first time, that cells infected with intracellular bacteria can be lysed by human cytotoxic T lymphocytes.

• Research Organization: Naval Medical Research Institute, Bethesda, MD
• OSTI ID: 5441828
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 139:12; ISSN JOIMA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CELL PROLIFERATION
CESIUM 137
CESIUM ISOTOPES
CHROMIUM 51
CHROMIUM ISOTOPES
CLONE CELLS
CONNECTIVE TISSUE CELLS
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ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GAMMA RADIATION
HETEROCYCLIC COMPOUNDS
IMMUNOLOGY
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
IONIZING RADIATIONS
IRRADIATION
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LABELLED COMPOUNDS
LETHAL IRRADIATION
LEUKOCYTES
LYMPHOCYTES
LYSIS
MAMMALS
MAN
MATERIALS
MICROORGANISMS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RADIATIONS
RADIOISOTOPES
RIBOSIDES
RICKETTSIAE
RICKETTSIAL DISEASES
SECRETION
SOMATIC CELLS
THYMIDINE
TOXICITY
TRITIUM COMPOUNDS
TUMOR CELLS
TYPHUS
VERTEBRATES
YEARS LIVING RADIOISOTOPES