Organization of the gene for human factor XI

Journal Article · Tue Nov 17 04:00:00 EST 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00397a004· OSTI ID:5437798

Asakai, R; Davie, E W; Chung, D W

Factor XI (plasma thromboplastin antecedent) is a plasma glycoprotein that participates in the early phase of blood coagulation. The gene for the human protein has been isolated from two different lambda phage genomic libraries. Four independent recombinant lambda phage carrying overlapping DNA inserts that coded for the entire gene for factor IX were isolated and characterized by restriction mapping, Southern blotting, and selective DNA sequencing to establish the number and location of the intro-exon boundaries. The gene for human factor IX was 23 kilobases in length and consisted of 15 exons (I-XV) and 14 introns (A-N). Exon I coded for the 5' untranslated region, and exon II coded for the signal peptide. The next eight exons (III-X) coded for the four tandem repeats of 90 or 91 amino acids that were present in the amino-terminal region of the mature protein. Each of these tandem repeats was coded by two exons that were interrupted by a single intron, and these introns were located in essentially the same position within each of the four tandem repeats. The carboxyl-terminal region of the protein, which contained the catalytic chain, was coded by five exons (XI-IV) that were interrupted by four introns. The last four introns were located in the same positions as those in the genes for human tissue plasminogen activator and human urokinase.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 5437798
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:23; ISSN BICHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ATP
BACTERIA
BACTERIOPHAGES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD COAGULATION FACTORS
BLOOD PLASMA
BODY FLUIDS
CARBOHYDRATES
COAGULANTS
DAYS LIVING RADIOISOTOPES
DNA
DNA POLYMERASES
DNA SEQUENCING
DRUGS
ENZYMES
ESCHERICHIA COLI
EVEN-ODD NUCLEI
GENES
GENETIC MAPPING
GLUCOPROTEINS
HEMATOLOGIC AGENTS
HEMOSTATICS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAPPING
MATERIALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
RADIOISOTOPES
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
THROMBOPLASTIN
TRANSFERASES
VIRUSES