Serine-324 of myosin's heavy chain is photoaffinity-labeled by 3 prime (2 prime )-O-(4-benzoylbenzoyl)adenosine triphosphate
- Washington State Univ., Pullman (USA)
A portion of the active site of rabbit skeletal myosin near the ribose ring of ATP can be labeled by the photoaffinity analogue 3{prime}(2{prime})-O-(4-benzoylbenzoyl)adenosine triphosphate (Bz{sub 2}ATP). The specificity of the photolabeling was assured by first trapping ({sup 14}C)Bz{sub 2}ATP at the active site by use of thiol cross-linking agents. Five radioactive peptides were isolated by high-performance liquid chromatography after extensive trypsin and subtilisin digestion of photolabeled myosin subfragment 1. Four of these peptides were sequenced by Edman techniques, and all originated from a region with the sequence Gly-Glu-Ile-Thr-Val-Pro-Ser-Ile-Asp-Asp-Gln, which corresponds to rabbit myosin heavy chain residues 312-328. The fifth labeled peptide had an amino acid composition appropriate for residues 312-328. Amino acid composition, radiochemical analysis, and sequence data indicate that Ser-324 is the major amino acid residue photolabeled by Bz{sub 2}ATP. Spectrophotometric evidence indicates that the benzophenone carbonyl group has inserted into a C-H bond from either the {alpha}- or {beta}-carbon of serine. These results place Ser-324 at a distance of 6-7 {angstrom} from the 3{prime}(2{prime}) ribose oxygens of ATP bound at the active site of myosin.
- OSTI ID:
- 5434862
- Journal Information:
- Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 28:9; ISSN 0006-2960; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
ATP
ATP-ASE
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYMES
GLOBULINS
HYDROLASES
HYDROLYSIS
HYDROXY ACIDS
LABELLED COMPOUNDS
LABELLING
LYSIS
MAMMALS
MOLECULAR STRUCTURE
MUSCLES
MYOSIN
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHOHYDROLASES
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RABBITS
RADIOCHEMICAL ANALYSIS
SERINE
SKELETON
SOLVOLYSIS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
ATP
ATP-ASE
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYMES
GLOBULINS
HYDROLASES
HYDROLYSIS
HYDROXY ACIDS
LABELLED COMPOUNDS
LABELLING
LYSIS
MAMMALS
MOLECULAR STRUCTURE
MUSCLES
MYOSIN
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHOHYDROLASES
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RABBITS
RADIOCHEMICAL ANALYSIS
SERINE
SKELETON
SOLVOLYSIS
VERTEBRATES