Modulation of mitogenic stimuli by angiogenin correlates with in vitro phosphatidylinositol bisphosphate synthesis

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.8.2718· OSTI ID:5434154

Heath, Jr, W F; Moore, F; Bicknell, R; Vallee, B L ^[1]

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1. Harvard Medical School, Boston, MA (USA)

{sup 125}I-labeled angiogenin binds rapidly to the plasma membrane of several cell lines at 37{degree}C but in comparatively small amounts. Competition with unlabeled angiogenin varies markedly with different cell lines, being most effective in vascular smooth muscle and fibroblast cells. Angiogenin modulates mitogenic stimuli in bovine adrenal capillary endothelial (BACE), rat aortic smooth muscle (RASM), and fibroblast (3T3) cells. Thus, it enhances the mitogenic effect of certain conditioned media on RASM and 3T3 cells, but it inhibits the mitogenic effect on BACE cells. In RASM and 3T3 cells, mitogenesis is increased at low and high but not at intermediate concentrations of angiogenin. Plasma membranes from RASM and 3T3 cells that have been treated with angiogenin show an enhanced in vitro synthesis of phosphatidylinositol bisphosphate (PtdInsP{sub 2}) with an angiogenin concentration dependence similar to that of enhanced mitogenesis. PtdInsP{sub 2} synthesis arises by activation of a fatty acid (arachidonyl) coenzyme A synthetase and either a plasma membrane fatty acid acyltransferase or phosphatidylinositol kinase(s), or both. Increased PtdInsP{sub 2} or the derived second messengers (e.g., diacylglycerol) may mediate modulation of the mitogenic stimulus. The differential mitogenic interaction of angiogenin with several cell types, either stimulation or inhibition, probably reflects the multistep nature of angiogenesis.

• OSTI ID: 5434154
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:8; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
CROSS-LINKING
MITOGENS
PHOSPHOLIPIDS
BIOSYNTHESIS
BIOCHEMICAL REACTION KINETICS
BLOOD VESSELS
CAPILLARIES
DOSE-RESPONSE RELATIONSHIPS
IN VITRO
IODINE 125
MUSCLES
PROTEINS
TRITIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
LIPIDS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
POLYMERIZATION
RADIOISOTOPES
REACTION KINETICS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques