Accuracy of some routine method used in clinical chemistry as judged by isotope dilution-mass spectrometry

Journal Article · Fri May 01 00:00:00 EDT 1981 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5433098

Bjoerkhem, I; Bergman, A; Falk, O; Kallner, A; Lantto, O; Svensson, L; Akerloef, E; Blomstrand, R

Serum from patients was pooled, filtered, dispensed, and frozen. This pooled specimen was used for accuracy control in 64 participating laboratories in Sweden. Mean values (state-of-the-art values) were obtained for creatinine, cholesterol, glucose, urea, uric acid, and cortisol. These values were compared with values obtained with highly accurate reference methods based on isotope dilution-mass spectrometry. Differences were marked in the case of determination of creatinine and cortisol. Concerning the other components, the differences between the state-of-the-art value and the values obtained with the reference methods were negligible. Moreover, the glucose oxidase and the oxime methods for determination of glucose and urea were found to give significantly lower values than the hexokinase and urease methods, respectively. Researchers conclude that methods with a higher degree of accuracy are required for routine determination of creatinine and cortisol.

• OSTI ID: 5433098
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 27:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CREATININE
QUANTITATIVE CHEMICAL ANALYSIS
HYDROCORTISONE
MEASURING METHODS
ACCURACY
BLOOD SERUM
COMPARATIVE EVALUATIONS
ISOTOPE DILUTION
MASS SPECTROSCOPY
PATIENTS
ADRENAL HORMONES
AZOLES
CHEMICAL ANALYSIS
CORTICOSTEROIDS
GLUCOCORTICOIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
IMIDAZOLES
IMINES
ISOTOPE APPLICATIONS
KETONES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PREGNANES
SPECTROSCOPY
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques