A villous cell-derived inhibitor of intestinal cell proliferation

Journal Article · Tue Dec 01 04:00:00 EST 1981 · Am. J. Physiol.; (United States)

OSTI ID:5432974

May, R J ^[1]; Quaroni, A; Kirsch, K; Isselbacher, K J

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1. Harvard Medical School, Boston, MA

To test the hypothesis that the intestinal villous cell synthesizes a mitotic inhibitor that is specific for crypt cells, we have partially purified an extract from rat intestinal villous cells (VCE) and have demonstrated that it strongly and reversibly inhibits cell division and DNA synthesis in an intestinal epithelial cell line (IEC-6 cells). VCE produced a 60-70% inhibition of (/sup 3/H)thymidine incorporation into DNA and a similar magnitude of inhibition of labeling of nuclei in autoradiographic studies. This inhibition was not associated with cytotoxicity as assessed by the effect of VCE on /sup 51/Cr release, hexose or amino acid uptake, and protein synthesis. VCE appears specific for IEC-6 cells because it did not affect DNA synthesis in 10 other cell lines, and extracts derived from other cell lines and from colonic mucosa did not affect DNA synthesis in IEC-6 cells. VCE may represent a villous cell factor involved in the control of intestinal epithelial cell turnover in vivo.

• OSTI ID: 5432974
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 241:6; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
CELL DIVISION
CELL PROLIFERATION
CHROMIUM 51
CHROMIUM ISOTOPES
CRYPT CELLS
DIGESTIVE SYSTEM
DNA
ELECTRON CAPTURE RADIOISOTOPES
ENZYME INHIBITORS
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MITOSIS
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
RATS
RODENTS
SOMATIC CELLS
SYNTHESIS
TRITIUM COMPOUNDS
VERTEBRATES