Cadmium-binding proteins in the mussel, Mytilus edulis
Inducible cadmium-binding proteins (Cd-BP) in the mussel, Mytilus edulis, were resolved into two molecular weight components, designated Cd-BP/sub 10/ and Cd-BP/sub 20/, by gel-permeation chromatography on Sephadex G-75. Each of these two molecular weight components were further resolved into four subcomponents by DEAE-ion-exchange chromatography. All eight subcomponents bound cadmium and exhibited significant UV absorption at 254 nm and little absorption at 280 nm. Each subcomponent was purified and subjected to amino acid composition analysis. Two classes were identified, one having higher cysteine and lower glutamic acid contents compared to the other class. All subcomponents have a relatively high glycine content relative to mammalian metallothioneins. Although the Cd-BP/sub 20/ have apparent molecular weights almost twice the Cd-BP/sub 10/, the exact molecular relationship between these binding proteins is not known.
- Research Organization:
- Johns Hopkins Univ., Baltimore, MD
- OSTI ID:
- 5431373
- Journal Information:
- Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 65; ISSN EVHPA
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Related Subjects
560304* -- Chemicals Metabolism & Toxicology-- Invertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACID SEQUENCE
ANIMALS
AQUATIC ORGANISMS
BIOCHEMISTRY
CADMIUM
CHEMISTRY
CHROMATOGRAPHY
ELEMENTS
GEL PERMEATION CHROMATOGRAPHY
INVERTEBRATES
ION EXCHANGE CHROMATOGRAPHY
METALLOPROTEINS
METALS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
MOLLUSCS
MUSSELS
ORGANIC COMPOUNDS
PROTEINS
SEPARATION PROCESSES
SPECTRA
ULTRAVIOLET SPECTRA