Glutathione peroxidase activity in mouse whole blood as affected by variations in dietary protein and selenium levels

Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5430746

Choe, M; Kies, C

The effects of different protein and selenium levels on glutathione peroxidase activity in whole blood as a measure of selenium utilization by mice was investigated. 72, 21-day old, male mice (NIH Swiss) were fed rations containing 3 different levels of protein (10%, 15%, 25%) and 3 different levels of selenium (0.003 ppm, 0.2 ppm, 2 ppm) in all possible combinations according to a 3 x 3 factorial design. On the last day of the 3-week experiment, mice were sacrificed and blood was drawn. Glutathione peroxidase activity in whole blood was measured by a modification of the method of Paglia and Valentine (1967). Mean whole blood glutathione peroxidase activities and dietary protein level were negatively correlated in the mice receiving low selenium rations. In moderate and high selenium fed groups, highest glutathione peroxidase activities were found at the moderate protein level (15% of ration) and tended to be lower when either the low protein ration (10%) or high protein ration (25%) was fed. Glutathione peroxidase activities in both the moderate and high selenium fed animals were higher than were those of low selenium fed animals regardless of level of protein.

• Research Organization: Univ. of Nebraska, Lincoln
• OSTI ID: 5430746
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
DIET
DRUGS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GLUTATHIONE
MAMMALS
MATERIALS
MICE
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEPTIDES
PEROXIDASES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RODENTS
SELENIUM
SEMIMETALS
VERTEBRATES