Solubility and selective crystallization of lactose from solutions of its hydrolysis products glucose and galactose
A high degree of conversion is desired when lactose is hydrolyzed to glucose and galactose. This produces, however, a high concentration of galactose, which is inhibitory for the enzyme catalyst (beta-galactosidase). The inhibition can be reduced by limiting the conversion per pass over the enzyme (e.g. to ca. 50%), separating unconverted lactose from the reactor effluent, and recycling it to the reactor inlet. (This allows the overall conversion to be raised to ca. 80-90%). The solubilities of lactose, glucose, and galactose have been determined at various temperatures and for sugar mixtures having different concentrations and degrees of hydrolysis. Various cooling crystallizations have defined convenient and simple processes for the selective separation of lactose from its hydrolysis products.
- Research Organization:
- Technischchemisches Laboratorium ETH, CH-8092 Zurich, Switzerland
- OSTI ID:
- 5424915
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 25:6; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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ALDEHYDES
CARBOHYDRATES
CENTRIFUGATION
CHEMICAL REACTIONS
CRYSTALLIZATION
DECOMPOSITION
DISACCHARIDES
ENZYMATIC HYDROLYSIS
ENZYMES
GALACTOSE
GALACTOSIDASE
GLUCOSE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
LACTOSE
LYSIS
MIXING
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PHASE TRANSFORMATIONS
SACCHARIDES
SEPARATION PROCESSES
SOLUBILITY
SOLVOLYSIS
TEMPERATURE EFFECTS