Determination of useful lifetime of immobilized beta-galactosidase for hydrolysis of lactose in permeate obtained from ultrafiltration of cottage cheese whey

Conference · Wed Jan 01 00:00:00 EST 1986 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:5812564

Scott, T C; Hill, Jr, C G; Amundson, C H; Scott, C D ^[1]

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1. ed.

Beta-galactosidase immobolized on a porous silica carrier was used to effect the hydrolysis of lactose in permeate that results from the ultrafiltration of cottage cheese whey. Results obtained from analyzing kinetics of the immobilized enzyme have led to the formulation of a model that can be used to assess catalyst performance when utilizing a specified reactor operating strategy. The model predicts a lifetime of 90 days for the catalyst bed when starting production at 30 degrees and maintaining a constant production rate by raising the operating temperature as a function of time-on-stream. The useful lifetime of the catalyst was not significantly affected by the extent of lactose conversion carried out in the reactor. This model has the potential for use in optimization of reactor operating conditions for industrial applications of the immobilized enzyme.

• Research Organization: Oak Ridge National Lab., Oak Ridge, TN 37831, USA. Chemical Technology Div.
• OSTI ID: 5812564
• Report Number(s): CONF-860508-
• Journal Information: Biotechnol. Bioeng. Symp.; (United States), Vol. 17; Conference: 8. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN, USA, 13 May 1986
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
GALACTOSIDASE
IMMOBILIZED ENZYMES
PERFORMANCE TESTING
WHEY
ENZYMATIC HYDROLYSIS
SACCHARIFICATION
BIOREACTORS
CATALYSTS
CHEMICAL REACTION KINETICS
FOOD INDUSTRY
LACTOSE
MATHEMATICAL MODELS
OPTIMIZATION
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
INDUSTRIAL WASTES
INDUSTRY
KINETICS
LYSIS
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
REACTION KINETICS
SACCHARIDES
SOLVOLYSIS
TESTING
WASTES
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)