Determination of useful lifetime of immobilized beta-galactosidase for hydrolysis of lactose in permeate obtained from ultrafiltration of cottage cheese whey
- ed.
Beta-galactosidase immobolized on a porous silica carrier was used to effect the hydrolysis of lactose in permeate that results from the ultrafiltration of cottage cheese whey. Results obtained from analyzing kinetics of the immobilized enzyme have led to the formulation of a model that can be used to assess catalyst performance when utilizing a specified reactor operating strategy. The model predicts a lifetime of 90 days for the catalyst bed when starting production at 30 degrees and maintaining a constant production rate by raising the operating temperature as a function of time-on-stream. The useful lifetime of the catalyst was not significantly affected by the extent of lactose conversion carried out in the reactor. This model has the potential for use in optimization of reactor operating conditions for industrial applications of the immobilized enzyme.
- Research Organization:
- Oak Ridge National Lab., Oak Ridge, TN 37831, USA. Chemical Technology Div.
- OSTI ID:
- 5812564
- Report Number(s):
- CONF-860508-
- Journal Information:
- Biotechnol. Bioeng. Symp.; (United States), Journal Name: Biotechnol. Bioeng. Symp.; (United States) Vol. 17; ISSN BIBSB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
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BIOREACTORS
CARBOHYDRATES
CATALYSTS
CHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ENZYMATIC HYDROLYSIS
ENZYMES
FOOD INDUSTRY
GALACTOSIDASE
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
IMMOBILIZED ENZYMES
INDUSTRIAL WASTES
INDUSTRY
KINETICS
LACTOSE
LYSIS
MATHEMATICAL MODELS
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
OPTIMIZATION
ORGANIC COMPOUNDS
PERFORMANCE TESTING
REACTION KINETICS
SACCHARIDES
SACCHARIFICATION
SOLVOLYSIS
TEMPERATURE DEPENDENCE
TESTING
TIME DEPENDENCE
WASTES
WHEY