Coordinated modulation of albumin synthesis and mRNA levels in cultured hepatoma cells by hydrocortisone and cyclic AMP analogs

Journal Article · Wed Oct 10 00:00:00 EDT 1979 · J. Biol. Chem.; (United States)

OSTI ID:5424113

Brown, P C; Papaconstantinou, J

The treatment of Hepa-2 cells, a permanent mouse hepatoma cell line, for 72 h with hydrocortisone (10/sup -6/ M), N/sup 6/,O/sup 2/-dibutyryl cyclic AMP (10/sup -3/ M), or 8-bromo cyclic AMP(10/sup -3/ M) results in a 2-, 3-, or 4-fold increase, respectively, in rates of synthesis and secretion of mouse serum albumin. Simultaneous treatment with hydrocortisone and N/sup 6/,O/sup 2/-dibutyryl cyclic AMP results in a 10-fold stimulation in these parameters, an effect that is significantly more than additive for the two compounds tested. The number of albumin mRNA sequences, determined by hybridization of total cell RNA to albumin complementary DNA, was increased in direct proportion to the increases in albumin synthesis in all experiments. The relative rate of albumin synthesis approaches in vivo levels in cells treated simultaneously with hydrocortisone and N/sup 6/,O/sup 2/-dibutyryl cyclic AMP. We propose that these factors may be necessary to maintain the maximal level of differentiated function in the continuous culture of Hepa-2 cells.

• Research Organization: Univ. of Tennessee, Oak Ridge
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5424113
• Journal Information: J. Biol. Chem.; (United States), Vol. 254:19
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
BIOSYNTHESIS
AMP
BIOLOGICAL EFFECTS
HYDROCORTISONE
CELL CULTURES
HEPATOMAS
MESSENGER-RNA
MICE
ADRENAL HORMONES
ANIMALS
CORTICOSTEROIDS
DISEASES
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
MAMMALS
NEOPLASMS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PREGNANES
PROTEINS
RNA
RODENTS
STEROID HORMONES
STEROIDS
SYNTHESIS
VERTEBRATES
550200* - Biochemistry