Effect of ozone and histamine on airway permeability to horseradish peroxidase in guinea pigs

Journal Article · Wed Jan 01 04:00:00 EST 1986 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398609530853· OSTI ID:5420791

Miller, P D; Gordon, T; Warnick, M; Amdur, M O

Airway permeability was studied in groups of male guinea pigs at 2, 8, and 24 h after a 1-h exposure to 1 ppm ozone or at 2 h after a 1-h exposure to filtered air (control). Intratracheal administration of 2 mg horseradish peroxidase (HRP) was followed by blood sampling at 5-min intervals up to 30 min. The rate of appearance of HRP in plasma was significantly higher at 2 and 8 h after ozone exposure than that found in animals examined 2 h after air exposure or 24 h after ozone exposure. A dose of 0.12 mg/kg of subcutaneous histamine given after the 15 min blood sample significantly increased the already elevated permeability seen at 2 h post ozone, but had no effect on animals exposed to filtered air 2 h earlier or to ozone 24 h earlier. No difference was seen in the amount of subcutaneous radiolabeled histamine in the lungs of animals exposed 2 h earlier either to air or to ozone. These data indicate that a short-term exposure to ozone produced a reversible increase in respiratory epithelial permeability to HRP in guinea pigs. The potentiation of this increased permeability by histamine may be another manifestation of ozone-induced hyperreactivity.

• Research Organization: Massachusetts Institute of Technology, Cambridge
• OSTI ID: 5420791
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 1; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL TISSUES
ANIMALS
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
ENZYME ACTIVITY
ENZYMES
EPITHELIUM
GUINEA PIGS
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LUNGS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
OZONE
PERMEABILITY
PEROXIDASES
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
RODENTS
TISSUES
TRACHEA
VERTEBRATES