Effect of ozone on respiratory response of guinea pigs to histamine

Journal Article · Tue Jan 01 04:00:00 EST 1980 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398009529841· OSTI ID:5128261

Gordon, T; Amdur, M O

The effect of preexposure to ozone on the respiratory response to histamine was studied. The Amdur-Mead technique was used to measure respiratory mechanics in Charles River Hartley guinea pigs. Groups of animals were exposed for 1 h to filtered control air or ozone. Control measurements were made 1 1/2 h after exposure, followed by sc injection of 0.125 mg/kg histamine dihydrochloride. Respiratory measurements were continued at 2-min intervals for 16 min, at which time resistance and compliance had returned to control values. The 2-h interval between exposure and injection and the 0.125 mg/kg histamine were the optimum time interval and dose, respectively, as determined by appropriate time-response and dose-response studies. Although control values did not differ between air-exposed and ozone-exposed animals, animals preexposed to 0.1, 0.2, 0.4, and 0.8 ppM ozone had increases in resistance of 111, 110, 93, and 97% after histamine administration, compared to the air-exposed animals, which had increases of 86, 86, 67, and 46%, respectively. Whereas the only significant difference in resistance between air and ozone animals was at the 0.8-ppM level (p < 0.05), significant differences in compliance changes were found at all levels of ozone exposure. Ozone animals had compliance decreases of 40, 45, 46, and 46% after histamine injection, while their respective air controls decreased 24, 24, 27, and 31%.Exposures to ozone of 0.2 ppM for 4 h and 0.4 ppM for 2 h provided doses equal to that of 0.8 ppM for 1 h, yet no significant dose-time relationship was found. Lingering sensitivity to pharmacologically active agents is a subtle but important manifestation of ozone toxicity.

• Research Organization: Massachusetts Inst. of Tech., Cambridge
• OSTI ID: 5128261
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 6:1; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

551000 -- Physiological Systems
560305* -- Chemicals Metabolism &amp; Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMALS
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
DOSE-RESPONSE RELATIONSHIPS
GUINEA PIGS
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OZONE
RESPIRATORY SYSTEM
RODENTS
TOXICITY
VERTEBRATES