B2 bradykinin receptor-like binding in rat renomedullary interstitial cells
A particulate fraction from cultured rat renomedullary interstitial cells (RRIC) was prepared for bradykinin (BK) binding studies. Incubation of three radiolabeled BK analogs, (/sup 125/I-Tyr/sup 1/)kallidin, (/sup 125/I-Tyr/sup 5/)-BK, and (/sup 125/I-Tyr/sup 8/)-BK, with the particulate fraction resulted in degradation of these peptides. Assay conditions which prevented hydrolysis of these radiolabeled kinins were determined. Under these conditions, direct binding studies were performed with (/sup 125/I-Tyr/sup 1/)kallidin (T1K) as the radioligand. BK binding affinity, apparent K/sub assoc/ = 1.3 x 10/sup 9/ M/sup -1/, and specificity, determined with 51 BK analogs, were consistent with those expected of a B2 BK receptor. 17 references, 2 figures, 2 tables.
- Research Organization:
- Indiana Univ., Bloomington
- OSTI ID:
- 5411389
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 37:4; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
BRADYKININ
CELL CULTURES
CHEMICAL BONDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELECTRON CAPTURE RADIOISOTOPES
HYDROLYSIS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KININS
LABELLED COMPOUNDS
LIGANDS
LYSIS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
SOLVOLYSIS
TRACER TECHNIQUES
VERTEBRATES