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Evidence for a heat shock transcription factor-independent mechanism for heat shock induction of transcription in Saccharomyces cerevisiae

Journal Article · · Proceedings of the National Academy of Sciences of the United States of America; (United States)
;  [1]
  1. Univ. of California, Los Angeles (USA)
+ Show Author Affiliations

Transcription of the DNA damage-responsive gene, DDRA2, of Saccharomyces cerevisiae is activated by heat shock treatment as well as by mutagen/carcinogen exposure. Deletion analysis of upstream noncoding sequence indicated that sequences between approximately {minus}190 and {minus}140 base pairs were necessary for heat shock and DNA damage regulation of transcription. Fusion of this region to a CYC1-lacZ reporter gene demonstrated that the sequence between {minus}202 and {minus}165 base pairs was sufficient for basal level and heat shock-induced expression. This DNA sequence was unable to bind heat shock transcription factor as judged by binding competition experiments in vitro. These results indicate that yeast possesses a second, heat shock transcription factor-independent mechanism for activating transcription in response to thermal stress.

DOE Contract Number:
FC03-87ER60615
OSTI ID:
5410085
Journal Information:
Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:17; ISSN 0027-8424; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
560200 -- Thermal Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL ADAPTATION
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
EUMYCOTA
FUNGI
GENE REGULATION
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
PLASMIDS
PROTEINS
RADIOISOTOPES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STRUCTURAL CHEMICAL ANALYSIS
TEMPERATURE EFFECTS
TRANSCRIPTION FACTORS
YEASTS