Identification of transcriptional regulatory elements in human mitochondrial DNA by linker substitution analysis

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.9.3.1200· OSTI ID:5407165

Topper, J N; Clayton, D A ^[1]

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1. Stanford Univ., CA (USA). Dept. of Pathology

Human mitochondrial DNA contains two major promoters, one for transcription of each strand of the helix. Previous mapping and mutagenesis data have localized these regulatory elements and have suggested regions important to their function. In order to define, at high resolution, the sequences critical for accurate and efficient transcriptional initiation, a linker substitution analysis of the entire promoter region was performed. Each promoter was shown to consist of approximately 50 base pairs comprising two functionally distinct elements. These and previous data strongly support a mode of transcription initiation requiring minimal sequences surrounding the initiation sites that are likely interactive with core polymerase and upstream regulatory domains capable of binding a transcription factor that modulates the efficiency of transcription initiation. Furthermore, in at least one case, this upstream regulatory domain is capable of operating biodirectionally.

• OSTI ID: 5407165
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 9:3; ISSN 0270-7306; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CELL CONSTITUENTS
DNA SEQUENCING
GENE REGULATION
GENE REPRESSORS
GENETIC MAPPING
HUMAN POPULATIONS
MAPPING
MITOCHONDRIA
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANOIDS
POPULATIONS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TRANSCRIPTION FACTORS