Structural analysis and DNA binding of the HMG domains of the human mitochondrial transcription factor A

Gangelhoff, Todd A.; Mungalachetty, Purnima S.; Nix, Jay C.; Churchill, Mair E.  A.

doi:10.1093/nar/gkp157

Structural analysis and DNA binding of the HMG domains of the human mitochondrial transcription factor A

Journal Article · Fri Mar 20 00:00:00 EDT 2009 · Nucleic Acids Research

DOI:https://doi.org/10.1093/nar/gkp157· OSTI ID:1625461

Gangelhoff, Todd A. ^[1]; Mungalachetty, Purnima S. ^[2]; Nix, Jay C. ^[3]; Churchill, Mair E. A. ^[4]

Univ. of Colorado, Denver, CO (United States). Dept. of Pharmacology; Univ. of Colorado, Denver, CO (United States). Molecular Biology Program; DOE/OSTI
Univ. of Colorado, Denver, CO (United States). Dept. of Pharmacology
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Advanced Light Source (ALS). Molecular Biology Consortium. Beamline 4.2.2
Univ. of Colorado, Denver, CO (United States). Dept. of Pharmacology; Univ. of Colorado, Denver, CO (United States). Molecular Biology Program

The mitochondrial transcription factor A (mtTFA) is central to assembly and initiation of the mitochondrial transcription complex. Human mtTFA (h-mtTFA) is a dual high mobility group box (HMGB) protein that binds site-specifically to the mitochondrial genome and demarcates the promoters for recruitment of h-mtTFB1, h-mtTFB2 and the mitochondrial RNA polymerase. The stoichiometry of h-mtTFA was found to be a monomer in the absence of DNA, whereas it formed a dimer in the complex with the light strand promoter (LSP) DNA. Each of the HMG boxes and the C-terminal tail were evaluated for their ability to bind to the LSP DNA. Removal of the C-terminal tail only slightly decreased nonsequence specific DNA binding, and box A, but not box B, was capable of binding to the LSP DNA. The X-ray crystal structure of h-mtTFA box B, at 1.35 Å resolution, revealed the features of a noncanonical HMG box. Interactions of box B with other regions of h-mtTFA were observed. Together, these results provide an explanation for the unusual DNA-binding properties of box B and suggest possible roles for this domain in transcription complex assembly.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1625461

Journal Information:: Nucleic Acids Research, Journal Name: Nucleic Acids Research Journal Issue: 10 Vol. 37; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

References (65)

GCN4, a eukaryotic transcriptional activator protein, binds as a dimer to target DNA. Hope, I. A.; Struhl, K. The EMBO Journal, Vol. 6, Issue 9 https://doi.org/10.1002/j.1460-2075.1987.tb02573.x	journal	September 1987
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
Binding of Human Mitochondrial Transcription Factor A, an HMG Box Protein, to a Four-Way DNA Junction Ohno, Tetsuji; Umeda, Shuyo; Hamasaki, Naotaka Biochemical and Biophysical Research Communications, Vol. 271, Issue 2 https://doi.org/10.1006/bbrc.2000.2656	journal	May 2000
Main-chain Bond Lengths and Bond Angles in Protein Structures Laskowski, Roman A.; Moss, David S.; Thornton, Janet M. Journal of Molecular Biology, Vol. 231, Issue 4 https://doi.org/10.1006/jmbi.1993.1351	journal	June 1993
Addition of a 29 Residue Carboxyl-terminal Tail Converts a Simple HMG Box-containing Protein into a Transcriptional Activator Dairaghi, Daniel J.; Shadel, Gerald S.; Clayton, David A. Journal of Molecular Biology, Vol. 249, Issue 1 https://doi.org/10.1006/jmbi.1995.9889	journal	May 1995
The HMG-box: a versatile protein domain occurring in a wide variety of DNA-binding proteins Štros, M.; Launholt, D.; Grasser, K. D. Cellular and Molecular Life Sciences, Vol. 64, Issue 19-20 https://doi.org/10.1007/s00018-007-7162-3	journal	June 2007
Chromatin and cell death Bianchi, Marco E.; Manfredi, Angelo Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, Vol. 1677, Issue 1-3 https://doi.org/10.1016/j.bbaexp.2003.10.017	journal	March 2004
The S.cerevisiae Architectural HMGB Protein NHP6A Complexed with DNA: DNA and Protein Conformational Changes upon Binding Masse, James E.; Wong, Ben; Yen, Yi-Meng Journal of Molecular Biology, Vol. 323, Issue 2 https://doi.org/10.1016/s0022-2836(02)00938-5	journal	October 2002
The solution structure and dynamics of the DNA-binding domain of HMG-D from Drosophila melanogaster Jones, David NM; Searles, M. Alexandra; Shaw, Graeme L. Structure, Vol. 2, Issue 7 https://doi.org/10.1016/s0969-2126(00)00063-0	journal	July 1994
Nonsequence-specific DNA recognition: a structural perspective Murphy, Frank V.; Churchill, Mair EA Structure, Vol. 8, Issue 4 https://doi.org/10.1016/s0969-2126(00)00126-x	journal	April 2000
Structure of the A-Domain of HMG1 and Its Interaction with DNA as Studied by Heteronuclear Three- and Four-Dimensional NMR Spectroscopy Hardman, Colin H.; Broadhurst, R. William; Raine, Andrew R. C. Biochemistry, Vol. 34, Issue 51 https://doi.org/10.1021/bi00051a007	journal	December 1995
Sequence and organization of the human mitochondrial genome Anderson, S.; Bankier, A. T.; Barrell, B. G. Nature, Vol. 290, Issue 5806 https://doi.org/10.1038/290457a0	journal	April 1981
Structural basis for DNA bending by the architectural transcription factor LEF-1 Love, John J.; Li, Xiang; Case, David A. Nature, Vol. 376, Issue 6543 https://doi.org/10.1038/376791a0	journal	August 1995
Human mitochondrial transcription factor B1 methylates ribosomal RNA at a conserved stem-loop Seidel-Rogol, Bonnie L.; McCulloch, Vicki; Shadel, Gerald S. Nature Genetics, Vol. 33, Issue 1 https://doi.org/10.1038/ng1064	journal	December 2002
Mitochondrial DNA mutations in human disease Taylor, Robert W.; Turnbull, Doug M. Nature Reviews Genetics, Vol. 6, Issue 5 https://doi.org/10.1038/nrg1606	journal	May 2005
The mitochondrial RNA polymerase contributes critically to promoter specificity in mammalian cells Gaspari, Martina; Falkenberg, Maria; Larsson, Nils-Göran The EMBO Journal, Vol. 23, Issue 23 https://doi.org/10.1038/sj.emboj.7600465	journal	November 2004
The N-terminal tail of histone H2A binds to two distinct sites within the nucleosome core Lee, K. -M.; Hayes, J. J. Proceedings of the National Academy of Sciences, Vol. 94, Issue 17 https://doi.org/10.1073/pnas.94.17.8959	journal	August 1997
Mitochondrial DNA mutations in human disease Greaves, Laura; Taylor, Robert IUBMB Life (International Union of Biochemistry and Molecular Biology: Life), Vol. 58, Issue 3 https://doi.org/10.1080/15216540600686888	journal	March 2006
The HMG-box mitochondrial transcription factor xl-mtTFA binds DNA as a tetramer to activate bidirectional transcription Antoshechkin, I. The EMBO Journal, Vol. 16, Issue 11 https://doi.org/10.1093/emboj/16.11.3198	journal	June 1997
The structure of a chromosomal high mobility group protein-DNA complex reveals sequence-neutral mechanisms important for non-sequence-specific DNA recognition Murphy IV, F. V. The EMBO Journal, Vol. 18, Issue 23 https://doi.org/10.1093/emboj/18.23.6610	journal	December 1999
Mitochondrial transcription factor A regulates mtDNA copy number in mammals Ekstrand, M. I. Human Molecular Genetics, Vol. 13, Issue 9 https://doi.org/10.1093/hmg/ddh109	journal	March 2004
The C-terminal Tail of Mitochondrial Transcription Factor A Markedly Strengthens its General Binding to DNA Ohgaki, Kippei; Kanki, Tomotake; Fukuoh, Atsushi The Journal of Biochemistry, Vol. 141, Issue 2 https://doi.org/10.1093/jb/mvm020	journal	December 2006
The DNA supercoiling architecture induced by the transcription factor xUBF requires three of its five HMG-boxes Stefanovsky, V. Nucleic Acids Research, Vol. 24, Issue 16 https://doi.org/10.1093/nar/24.16.3208	journal	August 1996
The role of intercalating residues in chromosomal high-mobility-group protein DNA binding, bending and specificity Klass, J. Nucleic Acids Research, Vol. 31, Issue 11 https://doi.org/10.1093/nar/gkg389	journal	June 2003
SuperPose: a simple server for sophisticated structural superposition Maiti, R.; Van Domselaar, G. H.; Zhang, H. Nucleic Acids Research, Vol. 32, Issue Web Server https://doi.org/10.1093/nar/gkh477	journal	July 2004
Relative abundance of the human mitochondrial transcription system and distinct roles for h-mtTFB1 and h-mtTFB2 in mitochondrial biogenesis and gene expression Cotney, Justin; Wang, Zhibo; Shadel, Gerald S. Nucleic Acids Research, Vol. 35, Issue 12 https://doi.org/10.1093/nar/gkm424	journal	June 2007
The splice variants of UBF differentially regulate RNA polymerase I transcription elongation in response to ERK phosphorylation Stefanovsky, Victor Y.; Moss, Tom Nucleic Acids Research, Vol. 36, Issue 15 https://doi.org/10.1093/nar/gkn484	journal	August 2008
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/s0907444904019158	journal	November 2004
Automated MAD and MIR structure solution Terwilliger, Thomas C.; Berendzen, Joel Acta Crystallographica Section D Biological Crystallography, Vol. 55, Issue 4 https://doi.org/10.1107/s0907444999000839	journal	April 1999
Architectural Transcription Factors Wolffe, Alan P. Science, Vol. 264, Issue 5162 https://doi.org/10.1126/science.8178167	journal	May 1994
Human Mitochondrial Transcription Factor B1 Interacts with the C-Terminal Activation Region of h-mtTFA and Stimulates Transcription Independently of Its RNA Methyltransferase Activity McCulloch, Vicki; Shadel, Gerald S. Molecular and Cellular Biology, Vol. 23, Issue 16 https://doi.org/10.1128/mcb.23.16.5816-5824.2003	journal	August 2003
Architectural Role of Mitochondrial Transcription Factor A in Maintenance of Human Mitochondrial DNA Kanki, T.; Ohgaki, K.; Gaspari, M. Molecular and Cellular Biology, Vol. 24, Issue 22 https://doi.org/10.1128/mcb.24.22.9823-9834.2004	journal	October 2004
Purification and Characterization of Human Mitochondrial Transcription Factor 1 Fisher, Robert P.; Clayton, David A. Molecular and Cellular Biology, Vol. 8, Issue 8 https://doi.org/10.1128/mcb.8.8.3496-3509.1988	journal	August 1988
Replication and Transcription of Vertebrate Mitochondrial DNA Clayton, David A. Annual Review of Cell Biology, Vol. 7, Issue 1 https://doi.org/10.1146/annurev.cb.07.110191.002321	journal	November 1991
A Mitochondrial Paradigm of Metabolic and Degenerative Diseases, Aging, and Cancer: A Dawn for Evolutionary Medicine Wallace, Douglas C. Annual Review of Genetics, Vol. 39, Issue 1 https://doi.org/10.1146/annurev.genet.39.110304.095751	journal	December 2005
Evidence for an Early Gene Duplication Event in the Evolution of the Mitochondrial Transcription Factor B Family and Maintenance of rRNA Methyltransferase Activity in Human mtTFB1 and mtTFB2 Cotney, Justin; Shadel, Gerald S. Journal of Molecular Evolution, Vol. 63, Issue 5 https://doi.org/10.1007/s00239-006-0075-1	journal	October 2006
Molecular basis of human 46X,Y sex reversal revealed from the three-dimensional solution structure of the human SRY-DNA complex Werner, Milton H.; Huth, Jeffrey R.; Gronenborn, Angela M. Cell, Vol. 81, Issue 5 https://doi.org/10.1016/0092-8674(95)90532-4	journal	June 1995
Size-Distribution Analysis of Proteins by Analytical Ultracentrifugation: Strategies and Application to Model Systems Schuck, Peter; Perugini, Matthew A.; Gonzales, Noreen R. Biophysical Journal, Vol. 82, Issue 2 https://doi.org/10.1016/S0006-3495(02)75469-6	journal	February 2002
In organello footprinting. Analysis of protein binding at regulatory regions in bovine mitochondrial DNA. Ghivizzani, S. C.; Madsen, C. S.; Hauswirth, W. W. Journal of Biological Chemistry, Vol. 268, Issue 12 https://doi.org/10.1016/S0021-9258(18)52928-8	journal	April 1993
DNA wrapping and bending by a mitochondrial high mobility group-like transcriptional activator protein. Fisher, R. P.; Lisowsky, T.; Parisi, M. A. Journal of Biological Chemistry, Vol. 267, Issue 5 https://doi.org/10.1016/S0021-9258(19)50739-6	journal	February 1992
Mitochondrial transcription initiation. Variation and conservation Shadel, G. S.; Clayton, D. A. Journal of Biological Chemistry, Vol. 268, Issue 22 https://doi.org/10.1016/S0021-9258(19)85387-5	journal	August 1993
The S.cerevisiae Architectural HMGB Protein NHP6A Complexed with DNA: DNA and Protein Conformational Changes upon Binding Masse, James E.; Wong, Ben; Yen, Yi-Meng Journal of Molecular Biology, Vol. 323, Issue 2 https://doi.org/10.1016/S0022-2836(02)00938-5	journal	October 2002
HMG1 and 2, and related ‘architectural’ DNA-binding proteins Thomas, Jean O.; Travers, Andrew A. Trends in Biochemical Sciences, Vol. 26, Issue 3 https://doi.org/10.1016/S0968-0004(01)01801-1	journal	March 2001
The solution structure and dynamics of the DNA-binding domain of HMG-D from Drosophila melanogaster Jones, David NM; Searles, M. Alexandra; Shaw, Graeme L. Structure, Vol. 2, Issue 7 https://doi.org/10.1016/S0969-2126(00)00063-0	journal	July 1994
Nonsequence-specific DNA recognition: a structural perspective Murphy, Frank V.; Churchill, Mair EA Structure, Vol. 8, Issue 4 https://doi.org/10.1016/S0969-2126(00)00126-X	journal	April 2000
Initiation and Beyond: Multiple Functions of the Human Mitochondrial Transcription Machinery Bonawitz, Nicholas D.; Clayton, David A.; Shadel, Gerald S. Molecular Cell, Vol. 24, Issue 6 https://doi.org/10.1016/j.molcel.2006.11.024	journal	December 2006
Basis for recognition of cisplatin-modified DNA by high-mobility-group proteins Ohndorf, Uta-Maria; Rould, Mark A.; He, Qing Nature, Vol. 399, Issue 6737 https://doi.org/10.1038/21460	journal	June 1999
The ABcs of mitochondrial transcription Shoubridge, Eric A. Nature Genetics, Vol. 31, Issue 3 https://doi.org/10.1038/ng0702-227	journal	July 2002
Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA Falkenberg, Maria; Gaspari, Martina; Rantanen, Anja Nature Genetics, Vol. 31, Issue 3 https://doi.org/10.1038/ng909	journal	June 2002
Functional analysis of DNA bending and unwinding by the high mobility group domain of LEF-1 Giese, K.; Pagel, J.; Grosschedl, R. Proceedings of the National Academy of Sciences, Vol. 94, Issue 24 https://doi.org/10.1073/pnas.94.24.12845	journal	November 1997
Protein Components of Mitochondrial DNA Nucleoids in Higher Eukaryotes Bogenhagen, Daniel F.; Wang, Yousong; Shen, Ellen L. Molecular & Cellular Proteomics, Vol. 2, Issue 11 https://doi.org/10.1074/mcp.M300035-MCP200	journal	November 2003
The Mitochondrial Transcription Factor TFAM Coordinates the Assembly of Multiple DNA Molecules into Nucleoid-like Structures Kaufman, Brett A.; Durisic, Nela; Mativetsky, Jeffrey M. Molecular Biology of the Cell, Vol. 18, Issue 9 https://doi.org/10.1091/mbc.E07-05-0404	journal	September 2007
DNA looping in the RNA polymerase I enhancesome is the result of non-cooperative in-phase bending by two UBF molecules Stefanovsky, V. Y. Nucleic Acids Research, Vol. 29, Issue 15 https://doi.org/10.1093/nar/29.15.3241	journal	August 2001
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Automated MAD and MIR structure solution Terwilliger, Thomas C.; Berendzen, Joel Acta Crystallographica Section D Biological Crystallography, Vol. 55, Issue 4 https://doi.org/10.1107/S0907444999000839	journal	April 1999
Solution structure and backbone dynamics of the DNA-binding domain of mouse Sox-5 Cary, P. D. Protein Science, Vol. 10, Issue 1 https://doi.org/10.1110/ps.32801	journal	January 2001
Use of high-performance size-exclusion chromatography to measure protein molecular weight and hydrodynamic radius: An investigation of the properties of the TSK 3000 SW column Tarvers, Richard C.; Church, Frank C. International Journal of Peptide and Protein Research, Vol. 26, Issue 5 https://doi.org/10.1111/j.1399-3011.1985.tb01021.x	journal	November 1985
Short-range DNA looping by the Xenopus HMG-box transcription factor, xUBF Bazett-Jones, D.; Leblanc, B.; Herfort, M. Science, Vol. 264, Issue 5162 https://doi.org/10.1126/science.8178172	journal	May 1994
A Human Mitochondrial Transcription Factor Is Related to RNA Adenine Methyltransferases and Binds S-Adenosylmethionine McCulloch, Vicki; Seidel-Rogol, Bonnie L.; Shadel, Gerald S. Molecular and Cellular Biology, Vol. 22, Issue 4 https://doi.org/10.1128/MCB.22.4.1116-1125.2002	journal	February 2002
Human Mitochondrial Transcription Factor B1 Interacts with the C-Terminal Activation Region of h-mtTFA and Stimulates Transcription Independently of Its RNA Methyltransferase Activity McCulloch, Vicki; Shadel, Gerald S. Molecular and Cellular Biology, Vol. 23, Issue 16 https://doi.org/10.1128/MCB.23.16.5816-5824.2003	journal	August 2003
Architectural Role of Mitochondrial Transcription Factor A in Maintenance of Human Mitochondrial DNA Kanki, T.; Ohgaki, K.; Gaspari, M. Molecular and Cellular Biology, Vol. 24, Issue 22 https://doi.org/10.1128/MCB.24.22.9823-9834.2004	journal	October 2004
Purification and characterization of human mitochondrial transcription factor 1. Fisher, R. P.; Clayton, D. A. Molecular and Cellular Biology, Vol. 8, Issue 8 https://doi.org/10.1128/MCB.8.8.3496	journal	August 1988
Structure of the A-Domain of HMG1 and Its Interaction with DNA As Studied by Heteronuclear Three- and Four-Dimensional NMR Spectroscopy Hardman, Colin H.; Broadhurst, R. William; Raine, Andrew R. C. Biological Magnetic Resonance Bank https://doi.org/10.13018/bmr4079	dataset	January 2001
Solution Structure and Backbone Dynamics of the DNA-Binding Domain of Mouse Sox-5 Cary, P. D.; Read, C. M.; Davis, B. Biological Magnetic Resonance Bank https://doi.org/10.13018/bmr5036	dataset	January 2001
Expression and Maintenance of Mitochondrial DNA Shadel, Gerald S. The American Journal of Pathology, Vol. 172, Issue 6 https://doi.org/10.2353/ajpath.2008.071163	journal	June 2008

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