Amplification of bovine papillomavirus DNA by N-methyl-N'-nitro-N-nitrosoguanidine, ultraviolet irradiation, or infection with herpes simplex virus

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Virology; (USA)

DOI:https://doi.org/10.1016/0042-6822(89)90108-6· OSTI ID:5406903

Schmitt, J; Schlehofer, J R; Mergener, K; Gissmann, L; zur Hausen, H ^[1]

---

1. Deutsches Krebsforschungszentrum, Heidelberg (Germany, F.R.)

Treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or irradiation with ultraviolet light (uv254 nm) induces amplification of integrated as well as episomal sequences of bovine papillomavirus (BPV) type 1 DNA in BPV-1-transformed mouse C127 cells (i.e., ID13 cells). This is shown by filter in situ hybridization and Southern blot analysis of cellular DNA. Similarly, infection of ID13 cells with herpes simplex virus (HSV) type 1 which has been shown to be mutagenic for host cell DNA leads to amplification of BPV DNA sequences. In contrast to this induction of DNA amplification by initiators, treatment of ID13 cells with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) does not result in increased synthesis of BPV DNA nor does TPA treatment modulate the initiator-induced DNA amplification. Similar to other cell systems infection with adeno-associated virus (AAV) type 2 inhibits BPV-1 DNA amplification irrespective of the inducing agent. In contrast to initiator-induced DNA amplification, treatment with carcinogen (MNNG) or tumor promoters or combination of MNNG and promoter of C127 cells prior to transformation by BPV-1 does not lead to an increase in the number of transformed foci. The induction of amplification of papillomavirus DNA by initiating agents possibly represents one of the mechanisms by which the observed synergism between papillomavirus infection and initiators in tumorigenesis might occur.

• OSTI ID: 5406903
• Journal Information: Virology; (USA), Journal Name: Virology; (USA) Vol. 172:1; ISSN VIRLA; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARCINOGENS
CATTLE
CELL CULTURES
CELL TRANSFORMATIONS
DISEASES
DNA HYBRIDIZATION
DOMESTIC ANIMALS
ELECTROMAGNETIC RADIATION
ESTERS
GENE AMPLIFICATION
HERPES SIMPLEX
HYBRIDIZATION
INFECTIOUS DISEASES
MAMMALS
MICE
MICROORGANISMS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PHORBOL ESTERS
RADIATION EFFECTS
RADIATIONS
RODENTS
RUMINANTS
SKIN DISEASES
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES
VIRUSES