Pentose pathway in human liver

Journal Article · Fri Jul 01 00:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.13.4682· OSTI ID:5406068

Magnusson, I; Chandramouli, V; Schumann, W C; Kumaran, K; Wahren, J; Landau, B R ^[1]

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1. Karolinska Institute at Huddinge Hospital, Stockholm (Sweden)

(1-{sup 14}C)Ribose and (1-{sup 14}C)glucose were given to normal subjects along with glucose loads (1 g per kg of body weight) after administration of diflunisal and acetaminophen, drugs that are excreted in urine as glucuronides. Distributions of {sup 14}C were determined in the carbons of the excreted glucoronides and in the glucose from blood samples drawn from hepatic veins before and after glucagon administration. Eighty percent or more of the {sup 14}C from (1-{sup 14}C)ribose incorporated into the glucuronic acid moiety of the glucuronides was in carbons 1 and 3, with less than 8% in carbon 2. In glucuronic acid from glucuronide excreted when (2-{sup 14}C)glucose was given, 3.5-8.1% of the {sup 14}C was in carbon 1, 2.5-4.3% in carbon 3, and more than 70% in carbon 2. These distributions are in accord with the glucuronides sampling the glucose unit of the glucose 6-phosphate pool that is a component of the pentose pathway and is intermediate in glycogen formation. It is concluded that the glucuronic acid conjugates of the drugs can serve as a noninvasive means of sampling hepatic glucose 6-phosphate. In human liver, as in animal liver, the classical pentose pathway functions, not the L-type pathway, and only a small percentage of the glucose is metabolized via the pathway.

• OSTI ID: 5406068
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:13; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BIOLOGICAL WASTES
BLOOD
BODY
BODY FLUIDS
CARBOHYDRATES
CARBON 14 COMPOUNDS
DIGESTIVE SYSTEM
DISTRIBUTION
FEMALES
GLANDS
GLUCOSE
HEXOSES
LABELLED COMPOUNDS
LIVER
MALES
MAMMALS
MAN
MATERIALS
MEN
METABOLISM
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PENTOSES
PRIMATES
SACCHARIDES
TISSUE DISTRIBUTION
URINE
VERTEBRATES
WASTES
WOMEN