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Estimation of infarct size by determination of myocardial 3H-tetracycline accumulation in the coronary ligated rat

Journal Article · · Res. Commun. Chem. Pathol. Pharmacol.; (United States)
OSTI ID:5404579
Myocardial infarction was produced in rats by ligation of the left coronary artery. Rats were injected with 3H-tetracycline (50 muCi/kg) i.p., 10 minutes prior to ligation. The rats were killed at various time periods after ligation and the myocardial accumulation of 3H-tetracycline determined in the left ventricle or in whole heart homogenates. CPK was also determined in the myocardium or serum. Myocardial 3H-tetracycline was not significantly elevated in the whole heart homogenates. However, there was a significant increase in 3H-tetracycline in the digests of the left ventricle 6 h post-infarct. This increase correlated very well with serum CPK activity. This suggest that this method could be a useful tool in the estimation of infarct size.
Research Organization:
Department of Pharmacodynamics and Toxicology, College of Pharmacy, University of Nebraska Medical Center, Omaha
OSTI ID:
5404579
Journal Information:
Res. Commun. Chem. Pathol. Pharmacol.; (United States), Journal Name: Res. Commun. Chem. Pathol. Pharmacol.; (United States) Vol. 34:1; ISSN RCOCB
Country of Publication:
United States
Language:
English