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Cytotoxicity and mutagenicity of dimethylnitrosamine in mammalian cells (CHO/HGPRT system): enhancement by calcium phosphate

Journal Article · · Environ. Mutagen.; (United States)
 [1]; ; ;
  1. Oak Ridge National Lab., TN
+ Show Author Affiliations
The cytotoxicity and mutagenicity of dimethylnitrosamine (DMN) was determined in the CHO/HGPRT system. Metabolic activation of the promutagen was achieved by use of a liver homogenate supernatant (S9) prepared from Aroclor 1254-induced Sprague-Dawley rats. The cytotoxic and mutagenic effects of DMN were enhanced by the inclusion of calcium chloride in the incubation mix, and this enhancement was dependent on the presence of sodium phosphate. Under conditions that yielded maximal activity (10 mM calcium chloride, 10 mM magnesium chloride, 50 mM sodium phosphate), an apparent calcium phospate precipitate was observed. DMN activity increased with increasing amounts of S9 protein over the range 0.3-3.0 mg/ml in the S9 mix and appeared to plateau at higher concentrations. The mutagenicity of DMN can be described as 110 mutants/10/sup 6/ cells per mM DMN per mg/ml S9 protein per hour.
DOE Contract Number:
W-7405-ENG-26
OSTI ID:
5400953
Journal Information:
Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 4:1; ISSN ENMUD
Country of Publication:
United States
Language:
English

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Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METAL COMPOUNDS
AMINES
ANIMAL CELLS
ANIMALS
BODY
CALCIUM COMPOUNDS
CALCIUM PHOSPHATES
DIGESTIVE SYSTEM
FEMALE GENITALS
GLANDS
GONADS
HAMSTERS
HOMOGENATES
LIVER
MAMMALS
METABOLIC ACTIVATION
MUTAGENESIS
NITROSAMINES
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OVARIES
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
RATS
RESPONSE MODIFYING FACTORS
RODENTS
VERTEBRATES