Expression of c-myc and induction of DNA synthesis by platelet-poor plasma in human diploid fibroblasts

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90138-3· OSTI ID:5394538

Ferrari, S; Calabretta, B; Battini, R; Cosenza, S C; Owen, T A; Soprano, K J; Baserga, R ^[1]

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1. Temple Univ. Medical School, Philadelphia, PA (USA)

When WI-38 human diploid fibroblasts become confluent, they stop synthesizing DNA and dividing. Addition of serum causes the quiescent cell to reenter the cell cycle. Prolonged quiescence after confluence decreases and delays the response to serum. For a few days after reaching confluence. WI-38 cells also respond to platelet-poor plasma. During this period, although not cycling, WI-38 cells still express c-myc and other growth-regulated genes, as measured by steady-state RNA levels. If the quiescence is prolonged further, c-myc expression (and that of two other growth-regulated genes) is no longer detectable, and its disappearance coincides with a loss of response to platelet-poor plasma. These results suggest that, also under physiological conditions, the expression of c-myc and other growth-regulated genes can cooperate with platelet-poor plasma in inducing cellular DNA synthesis in human diploid fibroblasts.

• OSTI ID: 5394538
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 174:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CELL CONSTITUENTS
CELL CULTURES
CHEMICAL ACTIVATION
CONNECTIVE TISSUE CELLS
DNA REPLICATION
FIBROBLASTS
GENES
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ONCOGENES
ORGANIC COMPOUNDS
PLASMIDS
PRIMATES
RNA
SOMATIC CELLS
VERTEBRATES