Chronologic analysis of variations in retinal damage in two strains of rats after short-term illumination

Journal Article · Mon Feb 01 04:00:00 EST 1982 · Invest. Ophthalmol. Visual Sci.; (United States)

OSTI ID:5394149

O'Steen, W K; Donnelly, J E

In order to determine whether an optimal period existed for evaluating retinal photodamage in rats, a chronologic analysis was made of quantifiable changes in the outer nuclear layer (ONL) and retinal thicknesses in Sprague-Dawley (SD) and Wag/Rij rats after a single, 24 hr exposure to fluorescent light. A gradual reduction in ONL and retinal thicknesses occurred between postexposure days 0 and 10 and appeared to stabilize by day 14 in both strains. The percent difference in both ONL and retinal thickness in unexposed and exposed rats was greater in Wag/Rij than in SD rats. That the superior hemisphere of the retina was more susceptible to photodamage than the inferior was substantiated, and the difference was greater in the Wag/Rij than in SD rats. This finding emphasizes a significant variability between rat strains and is possibly a manifestation of the slowly progressing genetic retinopathy in the Wag/Rij rats. Results indicated that the optimal period for assessing photodamage under these experimental conditions was not sooner than 10 to 14 days after exposure. Quantifiable analyses prior to that time would not provide an accurate evaluation of the total influence of a short-tern exposure period.

• Research Organization: Department of Anatomy, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC
• OSTI ID: 5394149
• Journal Information: Invest. Ophthalmol. Visual Sci.; (United States), Journal Name: Invest. Ophthalmol. Visual Sci.; (United States) Vol. 22:2; ISSN IOVSD
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
550900 -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL VARIABILITY
BIOLOGY
BODY
BODY AREAS
CYTOLOGY
DAILY VARIATIONS
ELECTROMAGNETIC RADIATION
EYES
FACE
FLUORESCENT LAMPS
GENETIC VARIABILITY
HEAD
LIGHT BULBS
MAMMALS
MORPHOLOGICAL CHANGES
ORGANS
PHOTOSENSITIVITY
RADIATIONS
RATS
RETINA
RODENTS
SENSE ORGANS
SENSITIVITY
VARIATIONS
VERTEBRATES
VISIBLE RADIATION