Acute and late effects of a single exposure of ionizing radiation on cultured human diploid cell populations
The normal human diploid cells cultured for various numbers of days were exposed to x rays from 0 to 600 R at a dose rate of 60 R/min under ambient conditions. The cells showed a dose-survival response in terms of colony formation with a shoulder, having an extrapolation number (n) of 2.5 and an exponential slope (D/sub 0/) of 110 R throughout all the passages in vitro. The dose vs life-span response, however, had no shoulder and a D/sub 0/ of 600 R. Since x irradiation produces an acute lethal effect leading to a reduction in the cell population size, there is a possibility that the additional doublings required to compensate for the reduction in population size may correspond to the decreased life-span in irradiated cultures. It was shown, however, that cells irradiated with x rays revealed reductions in life-span that were greater than those calculated from the acute lethal effects and, furthermore, the percentage of life-shortening induced in cells by x irradiation in vitro showed a change with age of the culture.
- Research Organization:
- Kyoto Univ. (Japan)
- OSTI ID:
- 5393989
- Journal Information:
- Radiat. Res.; (United States), Vol. 81:1
- Country of Publication:
- United States
- Language:
- English
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CELL CULTURES
RADIOSENSITIVITY
X RADIATION
BIOLOGICAL RADIATION EFFECTS
ANIMAL CELLS
COLONY FORMATION
DIPLOIDY
DOSE-RESPONSE RELATIONSHIPS
LIFE SPAN
POPULATION DYNAMICS
RADIATION DOSES
SURVIVAL CURVES
TIME DEPENDENCE
BIOLOGICAL EFFECTS
DOSES
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
PLOIDY
RADIATION EFFECTS
RADIATIONS
560121* - Radiation Effects on Cells- External Source- (-1987)
560151 - Radiation Effects on Animals- Man